MAGI-1 抗体 (SS-5): sc-100326

MAGI-1 Antibody (SS-5) is an IgG₁ κ mouse monoclonal MAGI-1 antibody (also designated BAP1 antibody, AIP3 antibody or MAGI1 antibody) that detects the MAGI-1 protein of mouse, rat and human origin by WB, IP, IF and ELISA. MAGI-1 Antibody (SS-5) is available as the non-conjugated anti-MAGI-1 antibody. The membrane-associated guanylate kinase (MAGUK) proteins are concentrated at the membrane-cytoskeletal interface where they facilitate the assembly of multiprotein complexes on the inner surface of the plasma membrane. Three protein-protein interaction modules characteristically define MAGUK related proteins: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. The closely related MAGUK proteins, MAGI-1, MAGI-2 and MAGI-3 (membrane associated guanylate kinase inverted-1 and 2), likewise contain the GuK domain and five PDZ domains; however, the SH3 domain is replaced with a WW domain. The transcripts of MAGI-1 are alternatively spliced to produce three distinct proteins having unique C-terminals. Two variants, MAGI-1a and MAGI-1b, are associated with the membrane and cytosolic fractions and are primarily expressed in the brain. The third isoform, MAGI-1c, encodes for a nuclear localization signal that localizes MAGI-1c to the nucleus, and it is primarily expressed in the liver and kidney. MAGI-2 and MAGI-3 are localized to the plasma membrane, and they contribute to protein scaffolding by associating with the protein phosphatase PTEN.

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References:

1. Signaling pathways are focused at specialized regions of the plasma membrane by scaffolding proteins of the MAGUK family.  |  Dimitratos, SD., et al. 1999. Bioessays. 21: 912-21. PMID: 10517864
2. Interaction of the tumor suppressor PTEN/MMAC with a PDZ domain of MAGI3, a novel membrane-associated guanylate kinase.  |  Wu, Y., et al. 2000. J Biol Chem. 275: 21477-85. PMID: 10748157
3. Evidence for regulation of the PTEN tumor suppressor by a membrane-localized multi-PDZ domain containing scaffold protein MAGI-2.  |  Wu, X., et al. 2000. Proc Natl Acad Sci U S A. 97: 4233-8. PMID: 10760291
4. MAGI-1 interacts with beta-catenin and is associated with cell-cell adhesion structures.  |  Dobrosotskaya, IY. and James, GL. 2000. Biochem Biophys Res Commun. 270: 903-9. PMID: 10772923
5. MAGI-1 is required for Rap1 activation upon cell-cell contact and for enhancement of vascular endothelial cadherin-mediated cell adhesion.  |  Sakurai, A., et al. 2006. Mol Biol Cell. 17: 966-76. PMID: 16339077
6. Cleavage of MAGI-1, a tight junction PDZ protein, by caspases is an important step for cell-cell detachment in apoptosis.  |  Gregorc, U., et al. 2007. Apoptosis. 12: 343-54. PMID: 17191119
7. Cell signalling: MAGUK magic.  |  Anderson, JM. 1996. Curr Biol. 6: 382-4. PMID: 8723338
8. MAGI-1, a membrane-associated guanylate kinase with a unique arrangement of protein-protein interaction domains.  |  Dobrosotskaya, I., et al. 1997. J Biol Chem. 272: 31589-97. PMID: 9395497
9. Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins.  |  Wood, JD., et al. 1998. Mol Cell Neurosci. 11: 149-60. PMID: 9647693