Ly-6H 抗体 (MN-7): sc-100285

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- Ly-6H 抗体 MN-7 是小鼠单克隆 IgG₁ (kappa轻链)，Ly-6H抗体，规格为100 µg/ml
- 免疫human物种的重组Ly-6H
- 推荐用于 human 来源的Ly-6H WB, IP, IF, IHC(P) 和 ELISA检测
- 关于如何获取Ly-6H (MN-7): sc-100285的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成Ly-6H 抗体 (MN-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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此外 Ly 抗体包括 Ly-49, Ly-49F, Ly-49I, Ly-6A, Ly-6C, Ly-6D, Ly-6E, Ly-6G, Ly-6H 和 Ly-6K
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

Ly-6H Antibody (MN-7) - Western Blotting - Image 33659

Ly-6H Antibody (MN-7) - Immunohistochemistry - Image 35961

Ly-6H Antibody (MN-7) - Western Blotting - Image 31440

Ly-6H Antibody (MN-7) - Western Blotting - Image 30925

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Ly-6H Antibody (MN-7) is an IgG₁ κ mouse monoclonal Ly-6H antibody (also designated Ly-6H antibody) that detects the Ly-6H protein of human origin by WB, IP, IF, IHC(P) and ELISA. Ly-6H Antibody (MN-7) is available as the non-conjugated anti-Ly-6H antibody. The Ly-6 (Lymphocyte antigen 6) alloantigens represent a family of phosphatidylinositol-anchored proteins that play a role in the process of T lymphocyte activation. Ly-6 family members share amino acid homology throughout a distinctive cysteine rich protein domain that incorporates O-linked carbohydrates. During hematopoiesis, murine Ly-6 molecules have unique patterns of tissue expression, from multipotential stem cells to lineage committed precursor cells, and on specific leukocyte subpopulations in the peripheral lymphoid tissues. Ly-6H (Lymphocyte antigen 6H), also known as NMLY6, is a 140 amino acid lipid-anchored membrane protein that belongs to the Ly-6 family. Highly expressed in brain with lower expression in pancreas, testis, colon and small intestine, Ly-6H participates in the activation of T lymphocytes. Overexpression of Ly-6H is detected in certain acute human leukemic cell lines (such as MOLT-3), suggesting a possible role for Ly-6H in carcinogenesis.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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References:

Identification of mouse Ly6H and its expression in normal tissue. | Apostolopoulos, J., et al. 1999. Immunogenetics. 49: 987-90. PMID: 10501842
Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion. | Flanagan, K., et al. 2008. J Immunol. 180: 3874-81. PMID: 18322195
The LY-6 locus: a multigene family encoding phosphatidylinositol-anchored membrane proteins concerned with T-cell activation. | Rock, KL., et al. 1989. Immunol Rev. 111: 195-224. PMID: 2697681
Isolation of a murine Ly-6 cDNA reveals a new multigene family. | LeClair, KP., et al. 1986. EMBO J. 5: 3227-34. PMID: 3028776
Isolation and characterization of a new member of the human Ly6 gene family (LY6H). | Horie, M., et al. 1998. Genomics. 53: 365-8. PMID: 9799603

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	LY6H	Entrez Gene ID: 4062	8q24.3	XM_017013413, XM_017013414, NM_001130478, NM_001135655, NM_002347	UniProt ID: O94772	603625

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗Ly-6H抗体(MN-7)在各种科学出版物中有0篇引文。

针对哺乳动物靶标蛋白的一抗，如抗Ly-6H 抗体 (MN-7)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

Ly-6H 抗体 (MN-7) Specifications

替代产品名称

Ly-6H

产品克隆名称

MN-7

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

26-141

抗原表位靶标

Ly-6H

抗原表位种属

human

产品反应性/检测

Ly-6H

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

0篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗Ly-6H抗体(MN-7)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

Can sc-100285: Ly-6H (MN-7) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11

Thank you for your question. Yes, sc-100285: Ly-6H (MN-7) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 5 out of 5 by KG from Clean blot with single strong band at expected Clean blot with single strong band at expected size using K-562 whole cell lysate. -SCBT QC

Date published: 2015-07-11

Rated 2 out of 5 by MC from WB of transfected lysate was a bit messy WB of transfected lysate was a bit messy but detected a band a expected mw -SCBT QC

Date published: 2015-04-25

Rated 5 out of 5 by ET from Produced superb immunoperoxidase membrane Produced superb immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded human tonsil tissue. -SCBT QC

Date published: 2014-06-22

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Ly-6H 抗体 (MN-7): sc-100285

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References:

Ly-6H 抗体 (MN-7) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

Can sc-100285: Ly-6H (MN-7) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?