LOXL2抗体 (3C5) 推荐用于 mouse, rat 和 human 来源的LOXL2 WB, IP 和 ELISA检测

LOXL2 抗体 (3C5): sc-293427

LOXL2抗体 (3C5) is rated 2.0 out of 5 by 3.
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Datasheets

    • 抗-LOXL2 抗体 3C5 是小鼠单克隆 IgG2b (kappa轻链),LOXL2抗体,规格为100 µg/ml
    • raised against amino acids 675-773 representing partial length LOXL2 of human origin
    • 推荐用于 mouse, rat 和 human 来源的LOXL2 WB, IP 和 ELISA检测
    • 关于如何获取LOXL2 (3C5): sc-293427免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • 目前,我们还没有完成LOXL2 抗体 (3C5)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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For Western Blot, is it recommended to use denatured or non-denatured conditions with LOXL2 (3C5): sc-293427 antibody?

Asked by: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 3 out of 5 by from Aspecific Bands I have used this Ab for Western Blot,1:1000, on MDA-MB-231 cells extracts. The signal is not well defined because there are a lot of specific bands although it is possible to clearly identified the protein of interest (LOXL2).
Date published: 2019-11-27
Rated 2 out of 5 by from Fair features Blocked in 5% BSA-TBST 1:500. Incubated in primary overnight in cold room. Tested on Murine Fallopian Tube Tissue and Human Ovarian Cell Lines.Worked in both but worked better on human cell lines and there are a lot of non-specific bands.
Date published: 2017-07-24
Rated 1 out of 5 by from No signal on western blots Tested this antibody twice in western blots with various tumor samples that vary from none to high levels of LOXL2 as determined by mass spectrometry; no signal detected in western blot when used in conjunction with SCBT Kappa-binding-HRP as secondary.
Date published: 2016-11-10
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