LMTK3抗体 (I-17) 推荐用于 mouse, rat 和 human 来源的LMTK3 WB, IP 和 ELISA检测

LMTK3 抗体 (I-17): sc-100418

LMTK3抗体 (I-17) is rated 3.3 out of 5 by 3.
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Datasheets

    • 抗-LMTK3 抗体 I-17 是小鼠单克隆 IgG1 (kappa轻链),LMTK3抗体,规格为50 µg/0.5 ml
    • raised against a partial recombinant protein mapping within amino acids 1151-1250 of LMTK3 of human origin
    • 推荐用于 mouse, rat 和 human 来源的LMTK3 WB, IP 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取LMTK3 (I-17): sc-100418免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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What is the recommended starting dilution for Western blot for LMTK3 (I-17): sc-100418 monoclonal antibodyy?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Tech Service
Date published: 2017-02-02
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Rated 1 out of 5 by from Erster Blot leider erfolglos Prima war, daß ich innerhalb 1 Woche eine kostenlose Probe des LMTK3 (I-17) Antikörpers zugeschickt bekam. Meine erster Blot blieb leider ohne Signal/nur Unspezifisches. Hatte 20µg Gesamtprotein geladen und nach unserem Standard-Protokoll geblottet. Werde aber noch nicht aufgeben, ggf. mehr Lysat nötig, bzw. Kernfraktion bzw. Änderung des Blottens nötig, da 154kDa doch schon ein höheres MW ist. Lt. Datasheet zeigt dieser Ab ja auch ein paar unspezifische Banden. Bleibe dran und bedanke mich trotzdem herzlich für die kostenlose Probe!
Date published: 2017-08-02
Rated 5 out of 5 by from Works great in western blot We use this antibody for western blot and we got a great band around the correct molecular weight
Date published: 2017-01-10
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