Ki67抗体 (MIB-1) 推荐用于 human 来源的Ki67 IF 和 IHC(P)检测

Ki67 抗体 (MIB-1): sc-101861

Ki67抗体 (MIB-1) is rated 4.0 out of 5 by 4.
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Datasheets

    • Ki67 抗体 MIB-1 是小鼠单克隆 IgG1, 在56篇文献中引用,提供 1 ml supernatant
    • 免疫自human来源的Hodgkin的淋巴瘤细胞系L248的核提取物。
    • 推荐用于 human 来源的Ki67 IF 和 IHC(P)检测
    • 看 Ki67 (Ki-67): sc-23900,可查看有标记的 Ki67 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
    • 目前,我们还没有完成Ki67 抗体 (MIB-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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What is the concentration of this antibody?

Asked by: MDW47
Thank you for your question. This antibody is provided as cultured supernatant and the exact concentration of IgG has not been determined. Please see our product data sheet for our recommended usage instructions under the applications.
Answered by: Technical Support
Date published: 2019-01-31

Is this Antibody cross react with mouse antigen? I am going to do Paraffin embedded tissue IHC.

Asked by: Jeongin
Thank you for your question. We do not currently recommend this antibody, Ki-67 (MIB-1): sc-101861, for detection of mouse Ki-67 because we have not yet tested the antibody for cross-reactivity with this species.
Answered by: Technical Support
Date published: 2017-06-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-08-30

Will this antibody react with bovine tissue

Asked by: immuno
Thank you for your question. It is unknown whether or not this antibody can react with bovine samples. Ki-67 Antibody (MIB-1): sc-101861 is raised against nuclear fractions of hodgkin's lymphoma cell line, L248 of human origin. The exact epitope recognized by this clone is unknown. Therefore, we can not predict cross-reactivity with bovine samples based on sequence homology. And we have not tested the antibody in bovine, nor are we aware of any publications in bovine. Unfortunately, we do not offer any Ki-67 antibodies at this time which are recommended for use in bovine.
Answered by: Technical Support
Date published: 2017-01-06
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Rated 5 out of 5 by from Reliable Antibody, Great for IHC This antibody is great for IF and tissue staining, high specificity and low background signal.
Date published: 2017-01-13
Rated 1 out of 5 by from Doesn't work for IHC at all Tried this antibody for immunohistochemistry FFPE tissues with known controls and didn't turn up.
Date published: 2016-12-04
Rated 5 out of 5 by from The antibody worked well in WB or IHC The antibody worked well in WB or IHC.
Date published: 2015-11-05
Rated 5 out of 5 by from Published IHC data using SC Published IHC data using SC-101861 with human soft tissue sections. -SCBT Publication Review
Date published: 2013-06-24
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