Kaptin 抗体 (3B1): sc-100918

Kaptin Antibody (3B1) is an IgG_2a κ mouse monoclonal Kaptin antibody (also designated Kaptin antibody) that detects the Kaptin protein of human origin by WB, IP, IF and ELISA. Kaptin Antibody (3B1) is available as the non-conjugated anti-Kaptin antibody. Platelets circulate in the blood as discoid cells which, when activated, change shape by polymerizing actin into several different structures, such as filopodia and stress fibers. Platelet activation is important for hemostasis. Kaptin (more commonly referred to as 2E4) binds to filamentous (F)-actin in an ATP-dependent manner and is involved in platelet activation. Kaptin is expressed in platelets, and localizes to the leading edge of the cells, supporting its involvement in the actin rearrangements that occur during activation. Kaptin is also present at the leading edge of the elongating stereocilium cells in the inner ear which play an integral role in the mechanotransduction of sound. Kaptin is involved in stereocilia formation, and may be an important factor in the development of DFNA4, a form of autosomal dominant non-syndromic hearing loss.

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References:

1. 2E4 (kaptin): a novel actin-associated protein from human blood platelets found in lamellipodia and the tips of the stereocilia of the inner ear.  |  Bearer, EL. and Abraham, MT. 1999. Eur J Cell Biol. 78: 117-26. PMID: 10099934
2. Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex.  |  Weed, SA., et al. 2000. J Cell Biol. 151: 29-40. PMID: 11018051
3. 2E4/Kaptin (KPTN)--a candidate gene for the hearing loss locus, DFNA4.  |  Bearer, EL., et al. 2000. Ann Hum Genet. 64: 189-96. PMID: 11409409
4. Actin dynamics in platelets.  |  Bearer, EL., et al. 2002. Int Rev Cytol. 217: 137-82. PMID: 12019562
5. Mechanisms of actin rearrangements mediating platelet activation.  |  Hartwig, JH. 1992. J Cell Biol. 118: 1421-42. PMID: 1325975
6. An actin-associated protein present in the microtubule organizing center and the growth cones of PC-12 cells.  |  Bearer, EL. 1992. J Neurosci. 12: 750-61. PMID: 1372044
7. Cytoskeletal domains in the activated platelet.  |  Bearer, EL. 1995. Cell Motil Cytoskeleton. 30: 50-66. PMID: 7728868