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The Insulin receptor substrate (IRS) proteins are key components in signaling from the Insulin receptor. IRS-4 is the most recently characterized member of the IRS family and has an undefined in vivo function. Phosphorylated IRS-4 associates with phosphatidylinositol 3-kinase (PI3-kinase), involved in Insulin-stimulated DNA synthesis, GH-induced tyrosine phosphorylation of IRS-4 and nuclear translocation of Stat5. IRS-4 also associates with IRAS which, when overexpressed, enhances IRS-4-dependent Insulin stimulation of PI3-kinase. The IRS-4 protein exhibits a limited fiber type specific expression in heart and skeletal muscle tissue and has not yet been detected in any mouse or primary human tissue. The absence of IRS-4 in mice causes mild defects in growth, reproduction and glucose homeostasis, while overexpression of IRS-4 increases basal PI3-kinase activity and Akt phosphorylation. Defects in IRS-4-null mice may result from a lower overall blood glucose concentration.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Which IRS4 epitope is recognized by the antibody? Is it N-terminal within the amino acids 3-342? Thank you.
Asked by: thegut
Thank you for your question. The exact epitope used to raise this monoclonal antibody has not been mapped so we are unable to provide this information. Please contact our Technical Service Department for further support. We are happy to help perform sequence analysis for you to answer any specific questions you have about the antibody reactivity.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30
For Western Blot, is it recommended to use denatured or non-denatured conditions with IRS-4 (RI-7): sc-100854 antibody?
Asked by: Trav11
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Rated 4 out of
Clean band in WB indicating a good antibodyClean band in WB indicating a good antibody to use especially in HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-03
Rated 5 out of
Sizable band obtained in human IRSSizable band obtained in human IRS-4 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-19
Rated 4 out of
Good publishable data on Western blot onGood publishable data on Western blot on human endothelial cells. -SCBT Publication Review
Date published: 2015-01-16
Rated 5 out of
Publishable Western blot and IP data usingPublishable Western blot and IP data using human and mouse embryonic kidney epithelial 293T cells and NIH 3T3s -SCBT Publication Review