IRE1 alpha抗体 (YB-17) 推荐用于 human 来源的IRE1α WB, IP, IF 和 ELISA检测

IRE1α 抗体 (YB-17): sc-100772

IRE1α抗体 (YB-17) is rated 5.0 out of 5 by 3.
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Datasheets

    • IRE1α抗体(YB-17)是小鼠单克隆IgG2a (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组IRE1α
    • 推荐用于 human 来源的IRE1α WB, IP, IF 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 看 IRE1α (B-12): sc-390960,可查看有标记的 IRE1α 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790

I am using IRE1α (YB-17): sc-100772 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good for WB Single band seen at an appropriate molecular weight for IRE1 alpha in Western Blot with HeLa whole cell lysates.
Date published: 2017-01-27
Rated 5 out of 5 by from Good specificity in WB in HeLa whole cell lysate Good specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-05-27
Rated 5 out of 5 by from Produced exceptional immunofluorescence membrane Produced exceptional immunofluorescence membrane and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-08
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