IP3KB 抗体 (J-15): sc-100385

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Datasheets

- IP3KB 抗体 J-15 是小鼠单克隆 IgG_2b κ，IP3KB抗体, 在1篇文献中引用，规格为100 µg/ml
- raised against amino acids 545-644 representing an internal region of IP3KB of human origin
- 推荐用于 human 来源的IP3KB WB, IP 和 ELISA检测
- 关于如何获取IP3KB (J-15): sc-100385的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成IP3KB 抗体 (J-15)的首选二抗检测试剂的鉴定。这项工作正在进行中。

SEE ALSO...

此外 IP3K 抗体包括 IP3KB
ChemCruz™ 化学试剂 IP3K Inhibitors 用于功能分析细胞对IP3K反应

IP3KB Antibody (J-15) - Western Blotting - Image 368229

IP3KB Antibody (J-15) - Western Blotting - Image 33761

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IP3KB Antibody (J-15) is an IgG_2b κ mouse monoclonal IP3KB antibody (also designated IP3KB antibody) suitable for the detection of the IP3KB protein of human origin by WB, IP and ELISA. IP3KB Antibody (J-15) is available as the non-conjugated anti-IP3KB antibody. Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) regulates the level of calcium within the cell by releasing calcium from intracellular stores. Ins(1,4,5)P3 is phosphorylated by inositol 1,4,5-trisphosphate 3-kinase (IP3K) to form inositol 1,3,4,5-tetrakisphosphate (Ins(1,4,5)P4), which is thought to regulate the influx of calcium across the plasma membrane. IP3K exists as three isoforms, IP3KA, B, and C. IP3KA, the most highly characterized isoform, is expressed in rat brain and testis. IP3KB is expressed in various rat tissues such as lung, thymus, testis, brain and heart. IP3K activity is stimulated in the presence of calmodulin via phosphorylation by cAMP-dependent protein kinase, protein kinase C, or calcium/calmodulin dependent protein kinase II and, subsequently, mediates the inositol phosphate signaling pathways.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

Inositol tetrakisphosphate as a second messenger: confusions, contradictions, and a potential resolution. | Irvine, RF. 1991. Bioessays. 13: 419-27. PMID: 1659392
Regulation of D-myo-inositol 1,4,5-trisphosphate 3-kinase by cAMP-dependent protein kinase and protein kinase C. | Sim, SS., et al. 1990. J Biol Chem. 265: 10367-72. PMID: 2162347
Cloning and expression in Escherichia coli of a rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase. | Takazawa, K., et al. 1990. Biochem J. 272: 107-12. PMID: 2176078
Inositol phosphates and cell signalling. | Berridge, MJ. and Irvine, RF. 1989. Nature. 341: 197-205. PMID: 2550825
Purification of D-myo-inositol 1,4,5-trisphosphate 3-kinase from rat brain. | Johanson, RA., et al. 1988. J Biol Chem. 263: 7465-71. PMID: 2836386
Tissue- and cell-specific expression of Ins(1,4,5)P3 3-kinase isoenzymes. | Vanweyenberg, V., et al. 1995. Biochem J. 306 (Pt 2): 429-35. PMID: 7887896
Expression, purification, and regulation of two isoforms of the inositol 1,4,5-trisphosphate 3-kinase. | Woodring, PJ. and Garrison, JC. 1997. J Biol Chem. 272: 30447-54. PMID: 9374536

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	ITPKB	Entrez Gene ID: 3707	1q42.12	XM_005273120, XM_017001211, NM_002221	UniProt ID: P27987	147522

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗IP3KB抗体(J-15)在各种科学出版物中有1篇引文。

针对哺乳动物靶标蛋白的一抗，如抗IP3KB 抗体 (J-15)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

IP3KB 抗体 (J-15) Specifications

替代产品名称

IP3KB

产品克隆名称

J-15

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG_2b kappa light chain

氨基酸

545-644

抗原表位靶标

IP3KB

抗原表位种属

human

产品反应性/检测

IP3KB

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, ELISA

瓶的信息

Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

1篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗IP3KB抗体(J-15)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

For Western Blot, is it recommended to use denatured or non-denatured conditions with IP3KB (J-15): sc-100385 antibody?

Asked by: DefinitelyNotMatt

Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.

Answered by: Technical Support

Date published: 2017-03-27

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IP3KB 抗体 (J-15): sc-100385

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References:

IP3KB 抗体 (J-15) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

For Western Blot, is it recommended to use denatured or non-denatured conditions with IP3KB (J-15): sc-100385 antibody?