IP-10抗体 (1) 推荐用于 human 来源的IP-10 WB, IF 和 IHC(P)检测

IP-10 抗体 (1): sc-101500

IP-10抗体 (1) is rated 4.3 out of 5 by 4.
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Datasheets

    • IP-10抗体(1)是小鼠单克隆IgG2b (kappa轻链),规格为200 µg/ml
    • 免疫human物种的重组IP-10
    • IP-10 抗体 (1) 推荐用于WB, IF 和 IHC(P),检测human 来源的 IP-10
    • 抗-IP-10 抗体 (1) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取IP-10 (1): sc-101500免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Is there any reported cross-reactivity with mouse? I'm looking for a CXCL10 antibody for IHC (P) on mouse tissue.

Asked by: meganMIT
Thank you for your question. There is no reported cross-reactivity with mouse using this antibody, IP-10 (1): sc-101500. We do offer an alternative antibody, IP-10 (E-2): sc-374092, which is cross-reactive with mouse. We have not yet tested this antibody for IHC(P), but there is a recent customer review indicating that this antibody worked well for them in IHC(P).
Answered by: Technical Support
Date published: 2018-03-01

How much sample do you recommend loading on the gel for Western blot detection using IP-10 (1): sc-101500?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from WB and worked fine Works well for WB with this product and good band were strongly shown.
Date published: 2017-05-20
Rated 5 out of 5 by from good effect Good membrane localization in formalin-fixed human brain tissue in IHC,Good effect.
Date published: 2017-03-10
Rated 5 out of 5 by from Produced publishable IHC data on human samples This IP-10 monoclonal antibody was featured in a handful of publications, used in WB, IHC and IHC(P) on human samples.
Date published: 2017-01-18
Rated 2 out of 5 by from Positive result in WB using human recombinant IP Positive result in WB using human recombinant IP-10. -SCBT QC
Date published: 2013-12-20
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