IDI1 抗体 (XY-7): sc-100550

IDI1 Antibody (XY-7) is an IgG₁ κ mouse monoclonal IDI1 antibody (also designated IDI1 antibody) that detects the IDI1 protein of human origin by WB, IP and ELISA. IDI1 Antibody (XY-7) is available as the non-conjugated anti-IDI1 antibody. IDI1 (isopentenyl-diphosphate delta isomerase 1), also known as IPP1 or IPPI1, is a 227 amino acid member of the IPP isomerase type I family and is involved in cholesterol biosynthesis. Localized to the peroxisome, IDI1 catalytically converts isopentenyl diphosphate (IPP) to its electrophilic isomer, dimethylallyl diphosphate (DMAPP). Specifically, IDI1 uses magnesium as a cofactor to catalyze the 1,3-allylic rearrangement of IPP, thus creating DMAPP, a substrate for subsequent reactions that synthesize farnesyl diphosphate and, ultimately, cholesterol. Defects in the gene encoding IDI1 may be associated with peroxisomal deficiency diseases, such as Zellweger syndrome, a congenital disorder caused by a reduction in the number of peroxisomes. Individuals affected with this disorder generally exhibit lack of muscle tone, an enlarged liver, mental retardation and, in some cases, death.

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References:

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2. IDI2, a second isopentenyl diphosphate isomerase in mammals.  |  Clizbe, DB., et al. 2007. J Biol Chem. 282: 6668-76. PMID: 17202134
3. The crystal structure of human isopentenyl diphosphate isomerase at 1.7 A resolution reveals its catalytic mechanism in isoprenoid biosynthesis.  |  Zheng, W., et al. 2007. J Mol Biol. 366: 1447-58. PMID: 17250851
4. A human promyelocyte mRNA transiently induced by TPA is homologous to yeast IPP isomerase.  |  Xuan, JW., et al. 1994. Genomics. 20: 129-31. PMID: 8020941
5. Farnesyl-diphosphate synthase is localized in peroxisomes.  |  Krisans, SK., et al. 1994. J Biol Chem. 269: 14165-9. PMID: 8188698
6. Human isopentenyl diphosphate: dimethylallyl diphosphate isomerase: overproduction, purification, and characterization.  |  Hahn, FM., et al. 1996. Arch Biochem Biophys. 332: 30-4. PMID: 8806705
7. Cloning and subcellular localization of hamster and rat isopentenyl diphosphate dimethylallyl diphosphate isomerase. A PTS1 motif targets the enzyme to peroxisomes.  |  Paton, VG., et al. 1997. J Biol Chem. 272: 18945-50. PMID: 9228075