IBRDC1 抗体 (36-A2): sc-101124

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- IBRDC1 抗体 36-A2 是小鼠单克隆 IgG_2a (kappa轻链)，IBRDC1抗体，规格为50 µg/0.5 ml
- 免疫human物种的重组IBRDC1
- 推荐用于 mouse, rat 和 human 来源的IBRDC1 WB, IP, IF 和 ELISA检测
- 目前，我们还没有完成IBRDC1 抗体 (36-A2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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IBRDC 抗体
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IBRDC1 Antibody (36-A2) - Immunofluorescence - Image 35804

IBRDC1 Antibody (36-A2) - Western Blotting - Image 34485

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IBRDC1 Antibody (36-A2) is an IgG_2a κ mouse monoclonal IBRDC1 antibody (also designated IBRDC1 antibody) suitable for the detection of the IBRDC1 protein of mouse, rat and human origin by WB, IP, IF and ELISA. IBRDC1 Antibody (36-A2) is available as the non-conjugated anti-IBRDC1 antibody. IBRDC1 (IBR domain-containing protein 1), also known as RNF217, is a 275 amino acid single pass membrane protein that contains two IBR (in between ring fingers)-type zinc finger motifs. It is a member of the RBR (ring between ring fingers) family of diverse proteins and belongs to the animal-specific I subfamily. RBR proteins participate in a wide variety of cellular events, including RNA metabolism, translation, transcription, cell-cycle control, cellular signaling, subcellular tethering and regulation of posttranslational modifications. IBRDC1 is believed to function as an E3 ubiquitin ligase that participates in protein ubiquitinylation and degradation.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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References:

Features of the parkin/ariadne-like ubiquitin ligase, HHARI, that regulate its interaction with the ubiquitin-conjugating enzyme, Ubch7. | Ardley, HC., et al. 2001. J Biol Chem. 276: 19640-7. PMID: 11278816
Parkin and relatives: the RBR family of ubiquitin ligases. | Marín, I., et al. 2004. Physiol Genomics. 17: 253-63. PMID: 15152079
Comparative genomics and protein domain graph analyses link ubiquitination and RNA metabolism. | Lucas, JI., et al. 2006. J Mol Biol. 357: 9-17. PMID: 16426638
UVPAR: fast detection of functional shifts in duplicate genes. | Arnau, V., et al. 2006. BMC Bioinformatics. 7: 174. PMID: 16569227
Structure of the Parkin in-between-ring domain provides insights for E3-ligase dysfunction in autosomal recessive Parkinson′s disease. | Beasley, SA., et al. 2007. Proc Natl Acad Sci U S A. 104: 3095-100. PMID: 17360614
The ring between ring fingers (RBR) protein family. | Eisenhaber, B., et al. 2007. Genome Biol. 8: 209. PMID: 17367545

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	RNF217	Entrez Gene ID: 154214	6q22.31	XM_011535494, XM_011535495, XM_011535496, XM_011535499, XM_011535500, XM_017010317, NM_001286398, NM_152553	UniProt ID: Q8TC41	n/a

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Protocols Online

I am using IBRDC1 (36-A2): sc-101124 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Trav11

Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 4 out of 5 by LR from Produced wonderful immunofluorescence cytoplasmic Produced wonderful immunofluorescence cytoplasmic staining in paraformaldehyde-fixed Hep G2 cells. -SCBT QC

Date published: 2015-03-06

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IBRDC1 抗体 (36-A2): sc-101124

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I am using IBRDC1 (36-A2): sc-101124 for immunofluorescence, what dilution of this antibody should I use?