HSP 56抗体 (329.1) 推荐用于 mouse, rat 和 human 来源的HSP 56 WB, IP 和 ELISA检测

HSP 56 抗体 (329.1): sc-100758

HSP 56抗体 (329.1) is rated 3.2 out of 5 by 5.
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Datasheets

    • 抗-HSP 56 抗体 329.1 是小鼠单克隆 IgG2a (kappa轻链),HSP 56抗体,规格为100 µg/ml
    • 免疫human物种的重组HSP 56
    • 推荐用于 mouse, rat 和 human 来源的HSP 56 WB, IP 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取HSP 56 (329.1): sc-100758免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Why does one blot show no band for HSP56 in untransfected 293T cells the other blot does, and why don't you see more of it when over expressed? These data do not convince me that this antibody is actually staining for HSP 56

Asked by: real or not
Thank you for your question. HSP proteins are generally pretty ubiquitously expressed so it is harder to show upregulation. The differences in signal observed between untransfected control 293T and HSP 56 transfected 293T could be a result of differences in protein loading, lysate preparations, exposure times, buffer quality, etc. There are many variables that can contribute to this observable difference. The data we show on our website is intended to be indicative of what you would expect when using this antibody. Please contact our Technical Service Department for further support.
Answered by: Technical Support
Date published: 2019-03-22

What is the molecular weight of HSP 56 ?

Asked by: TinTin
Thank you for your question. HSP 56 has an expected molecular weight of approximately 56 kDa. If you have any questions, please contact our Technical Service Department and we would be happy to assist you.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 2 out of 5 by from Use to detect HSP56 protein levels This antibody is very useful for protein detection in animal and human cell compartments. In particular, we used this antibody to detect protein levels in nuclear and cytosolic cell compartments in hippocampus and prefrontal cortex of mice and rats. In human samples we did not obtained protein bands detecting HSP56 levels. In general, we are not very satisfied with the obtained results, because Ab displayed low purity and high background in Western blot. Dilution to 1:200 for Western blot.
Date published: 2017-11-23
Rated 2 out of 5 by from Use to detect HSP56 protein levels This antibody is very useful for protein detection in animal and human cell compartments. In particular, we used this antibody to detect protein levels in nuclear and cytosolic cell compartments in hippocampus and prefrontal cortex of mice and rats. In human samples we did not obtained protein bands detecting HSP56 levels. In general, we are not very satisfied with the obtained results, because Ab displayed low purity and high background in Western blot. Dilution to 1:200 for Western blot.
Date published: 2017-11-23
Rated 4 out of 5 by from No band detected in U87 cell lysates (WB) No band detected for western blot with 25 ug U87 cell lysates at 1:1000 primary antibody dilution.
Date published: 2017-09-10
Rated 4 out of 5 by from Western blot analysis supporting specificity Western blot analysis supporting specificity of the antibody via of HSP 56 expression in non-transfected: sc-117752 and mouse HSP 56 transfected: sc-110358 293T whole cell lysates. -SCBT QC
Date published: 2015-03-30
Rated 4 out of 5 by from Supporting western blot analysis of HSP 56 Supporting western blot analysis of HSP 56 expression in HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-28
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