HPS-1抗体 (5G12G2) 推荐用于 mouse, rat 和 human 来源的HPS-1 WB, IP 和 ELISA检测

HPS-1 抗体 (5G12G2): sc-101435

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HPS-1 抗体 (5G12G2) is rated 5.0 out of 5 by 1.
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Datasheets

    • 抗-HPS-1 抗体 5G12G2 是小鼠单克隆 IgG2a (kappa轻链),HPS-1抗体,规格为200 µg/ml
    • 免疫human物种的重组HPS-1
    • 抗-HPS-1 抗体 (5G12G2) 推荐用于 WB, IP 和 ELISA,检测mouse, rat 和human 来源的 HPS-1
    • 抗-HPS-1 抗体 (5G12G2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取HPS-1 (5G12G2): sc-101435免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Hi, I am planning to perform a CRISPR K.O. cell line by NHEJ and Cas9n. I would like to do a WB to confirm my cell line does not produce functional protein. Thus I wonder where this antibody binds. Thanks for your help. Regards, Pepe

Asked by: JB2023
Thank you for your question. The epitope has not been mapped for this antibody, so we are unable to provide the exact antibody binding site. However, this will be fine to use for your purpose with CRISPR.
Answered by: Tech Service
Date published: 2019-05-10

For Western Blot, is it recommended to use denatured or non-denatured conditions with HPS-1 (5G12G2): sc-101435 monoclonal antibody?

Asked by: cjMara
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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