HORMAD1 抗体 (GG-Y): sc-101235

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Datasheets

- HORMAD1 抗体 GG-Y 是小鼠单克隆 IgG₁ (kappa轻链)，HORMAD1抗体，规格为100 µg/ml
- 免疫human物种的重组HORMAD1
- 推荐用于 human 来源的HORMAD1 WB, IP 和 ELISA检测
- 关于如何获取HORMAD1 (GG-Y): sc-101235的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成HORMAD1 抗体 (GG-Y)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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HORMAD 抗体
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HORMAD1 Antibody (GG-Y) - Western Blotting - Image 34594

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HORMAD1 Antibody (GG-Y) is an IgG₁ κ mouse monoclonal HORMAD1 antibody (also designated HORMAD1 antibody) that detects the HORMAD1 protein of human origin by WB, IP and ELISA. HORMAD1 Antibody (GG-Y) is available as the non-conjugated anti-HORMAD1 antibody. HORMAD1 (HORMA domain containing 1), also known as NOHMA (Newborn Ovary HORMA protein) or CT46 (Cancer/Testis antigen 46), is a 394 amino acid testis-specific protein. HORMAD1 contains one HORMA (Hop1p, Rev7p and MAD2) domain (a domain involved in chromatin binding) that makes up the entire full-length sequence of the protein. Proteins with HORMA domains are typically involved in modulating chromatin dynamics and structure. The HORMA domain is believed to act as an adaptor, recruiting other proteins to chromatin states that result from nonattachment to the mitotic spindle or from DNA double-strand breaks. HORMAD1 is a putative meiotic protein, as is suggested by its 25.8% homology with the yeast protein Hop1 (a meiosis-specific protein). In addition, HORMAD1 is overexpressed in a variety of carcinomas, including breast, lung, endometrial, colon, bladder and esophageal cancers.240.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

Restricted germ cell expression of a gene encoding a novel mammalian HORMA domain-containing protein. | Pangas, SA., et al. 2004. Gene Expr Patterns. 5: 257-63. PMID: 15567723
Identification of CT46/HORMAD1, an immunogenic cancer/testis antigen encoding a putative meiosis-related protein. | Chen, YT., et al. 2005. Cancer Immun. 5: 9. PMID: 15999985
Systematic search for gastric cancer-specific genes based on SAGE data: melanoma inhibitory activity and matrix metalloproteinase-10 are novel prognostic factors in patients with gastric cancer. | Aung, PP., et al. 2006. Oncogene. 25: 2546-57. PMID: 16331256
Integrated profiling of basal and luminal breast cancers. | Adεlaïde, J., et al. 2007. Cancer Res. 67: 11565-75. PMID: 18089785

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	HORMAD1	Entrez Gene ID: 84072	1q21.3	XM_006711570, XM_006711571, XM_011510053, XM_011510054, XM_011510055, XM_011510056, NM_001199829, NM_032132	UniProt ID: Q86X24	609824

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗HORMAD1抗体(GG-Y)在各种科学出版物中有0篇引文。

针对哺乳动物靶标蛋白的一抗，如抗HORMAD1 抗体 (GG-Y)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

HORMAD1 抗体 (GG-Y) Specifications

替代产品名称

HORMAD1

产品克隆名称

GG-Y

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

1-395

抗原表位靶标

HORMAD1

抗原表位种属

human

产品反应性/检测

HORMAD1

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, ELISA

瓶的信息

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

0篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗HORMAD1抗体(GG-Y)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

Can this be used for ChipSeq?

Asked by: Meiosis

Thank you for your question. This antibody has not been tested for ChipSeq, but it is recommended for WB, IP and ELISA use. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.

Answered by: Tech Service

Date published: 2017-10-26

For Western Blot, is it recommended to use denatured or non-denatured conditions with HORMAD1 (GG-Y): sc-101235 antibody?

Asked by: jerojero

Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.

Answered by: Technical Support

Date published: 2017-03-27

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HORMAD1 抗体 (GG-Y): sc-101235

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References:

HORMAD1 抗体 (GG-Y) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

Can this be used for ChipSeq?

For Western Blot, is it recommended to use denatured or non-denatured conditions with HORMAD1 (GG-Y): sc-101235 antibody?