Date published: 2025-9-6
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hnRNP A1 Antibody (4B10) - Western Blotting - Image 357410
hnRNP A1 抗体 (4B10): sc-32301. PC-3 (A) 和 MDA-MB-231 (B) 全细胞裂解液以及 MCF7 (C) 和 SW480 (D) 核提取物中 hnRNP A1 表达的 Western 印迹分析.
hnRNP A1 抗体 (4B10): sc-32301. PC-3 (A) 和 MDA-MB-231 (B) 全细胞裂解液以及 MCF7 (C) 和 SW480 (D) 核提取物中 hnRNP A1 表达的 Western 印迹分析.

hnRNP A1 抗体 (4B10): sc-32301

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说明书
  • hnRNP A1 抗体 4B10 是小鼠单克隆 IgG2a κ，hnRNP A1抗体, 在149篇文献中引用，规格为200 µg/ml
  • 针对全长部分纯化的hnRNP A1
  • hnRNP A1 抗体 (4B10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 hnRNP A1; 也和以下物种反应，包括： 和 bovine and canine
  • 抗hnRNP A1抗体（4B10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取hnRNP A1 (4B10): sc-32301的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 hnRNP A1 抗体 (4B10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与hnRNP A1 抗体 (4B10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 hnRNP 抗体 包括 hnRNP A, hnRNP A1, hnRNP A2, hnRNP B, hnRNP B1, hnRNP C1, hnRNP C2, hnRNP E1, hnRNP E2和hnRNP F

hnRNP A1抗体（4B10）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫 酶联免疫吸附试验（ELISA）。抗hnRNP A1抗体（4B10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。异质核糖核蛋白A1（hnRNP A1）在调节前mRNA处理方面发挥着关键作用，对于mRNA的稳定性和运输至关重要。hnRNP A1主要位于细胞核中，与前mRNA相互作用并影响剪接位点选择，从而调节基因表达。hnRNP A1与其他hnRNP蛋白和剪接因子形成复合物，这些复合物对于剪接体组装至关重要，而剪接体是负责从前mRNA中剪接出内含子的分子机器。hnRN P A1能够在细胞核和细胞质之间穿梭，参与mRNA代谢的各个阶段，因此成为转录后调控的关键角色。hnRNP A1参与mRNA的运输和稳定，这凸显了它在维持正常基因表达和细胞功能方面的重要性，尤其是在应对压力和发育过程中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

hnRNP A1 抗体 (4B10) 参考文献:

  1. 核前 mRNA 区隔：将释放的转录本运送到剪接因子库。  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
  2. 在细胞核和细胞质之间穿梭的 hnRNPs 之间的蛋白质相互作用。  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
  3. hnRNP A1在RNA代谢调节中并作为潜在的治疗靶点。  |  Feng, J., et al. 2022. Front Pharmacol. 13: 986409. PMID: 36339596
  4. 撤回说明：HnRNP A1介导的CCDC50选择性剪接通过ZNF395促进透明细胞肾细胞癌的进展。  |  Sun, G., et al. 2023. J Exp Clin Cancer Res. 42: 43. PMID: 36755337
  5. microRNA-195-5p/hnRNP A1轴通过调节癌细胞的迁移，促进肝细胞癌的进展。  |  Jung, M., et al. 2023. Biochem Biophys Res Commun. 686: 149183. PMID: 37926044
  6. hnRNP A1功能障碍可改变RNA剪接并导致多发性硬化症（MS）中的神经变性。  |  Salapa, HE., et al. 2024. Nat Commun. 15: 356. PMID: 38191621
  7. hnRNP A1、hnRNP A2B1和hnRNP K在tau病中失调，但与tau病理不共定位。  |  Kavanagh, T., et al. 2024. Brain Pathol. e13305. PMID: 39354671
  8. 一种新型低丰度 hnRNP 蛋白的分离与鉴定：A0.  |  Myer, VE. and Steitz, JA. 1995. RNA. 1: 171-82. PMID: 7585247
  9. hnRNP A1 蛋白的核定位结构域。  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
  10. 章鱼 RNP 蛋白家族的三个新成员。  |  Good, PJ., et al. 1993. Nucleic Acids Res. 21: 999-1006. PMID: 8451200
  11. 新型人类 RNA 结合蛋白的鉴定和特性分析。  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
  12. 颉颃物前核糖核酸剪接因子的组织特异性调控表达。  |  Hanamura, A., et al. 1998. RNA. 4: 430-44. PMID: 9630249

订购信息

产品名称产品编号规格价格数量收藏夹

hnRNP A1 抗体 (4B10)

sc-32301
200 µg/ml
$316.00

hnRNP A1 (4B10): m-IgG Fc BP-HRP 套装

sc-528456
200 µg Ab; 10 µg BP
$354.00

hnRNP A1 (4B10): m-IgGκ BP-HRP 套装

sc-520837
200 µg Ab, 40 µg BP
$354.00

hnRNP A1 抗体 (4B10) AC

sc-32301 AC
500 µg/ml, 25% agarose
$416.00

hnRNP A1 抗体 (4B10) HRP

sc-32301 HRP
200 µg/ml
$316.00

hnRNP A1 抗体 (4B10) FITC

sc-32301 FITC
200 µg/ml
$330.00

hnRNP A1 抗体 (4B10) PE

sc-32301 PE
200 µg/ml
$343.00

hnRNP A1 抗体 (4B10) Alexa Fluor® 488

sc-32301 AF488
200 µg/ml
$357.00

hnRNP A1 抗体 (4B10) Alexa Fluor® 546

sc-32301 AF546
200 µg/ml
$357.00

hnRNP A1 抗体 (4B10) Alexa Fluor® 594

sc-32301 AF594
200 µg/ml
$357.00

hnRNP A1 抗体 (4B10) Alexa Fluor® 647

sc-32301 AF647
200 µg/ml
$357.00

hnRNP A1 抗体 (4B10) Alexa Fluor® 680

sc-32301 AF680
200 µg/ml
$357.00

hnRNP A1 抗体 (4B10) Alexa Fluor® 790

sc-32301 AF790
200 µg/ml
$357.00

Do you offer a biotin labelled hnRNP A1 Antibody (4B10): sc-32301? If so, is that custom made and what is the timeline?

Asked by: Faika Mseeh
Thank you for your question. Please contact technical service directly to request a biotin conjugate of this antibody as a custom order product.
Answered by: Tech Support Europe
Date published: 2021-02-09

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-32301 PE) or fluoroscein (sc-32301 FITC), Alexa Fluor® 488 (sc-32301 AF488) or Alexa Fluor® 647 (sc-32301 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 12
Date published: 2017-01-10
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Rated 5 out of 5 by from Very good antibodyThe antibody worked very well, very good and clean signal on WB.
Date published: 2021-07-21
Rated 5 out of 5 by from works very well for WBI got this about month ago. It pretty works for lung cancer cell lines.
Date published: 2019-03-07
Rated 4 out of 5 by from Works well in immunostainingAntibody gives good signal for endogenous protein in human fibroblasts (1:100, methanol fixation). Some cytoplasmic staining in addition to strong nuclear staining.
Date published: 2018-04-04
Rated 5 out of 5 by from very good for immunostainingIt is a very good antibody for immunofluorescence.
Date published: 2017-10-26
Rated 5 out of 5 by from Works for WB of endogenousDetects a main band beneath 37 kDa at 1/200 on HEK293T cell lysate
Date published: 2017-09-13
Rated 4 out of 5 by from got a lot of isoformsWhen I used the hnRNP A1 antibody (1:500 dilution) for Western blotting after an IP, I ended up detecting a lot of bands which looks like the different isoforms. You can get a strong signal when doing a Western blot using total lysate.
Date published: 2017-08-25
Rated 5 out of 5 by from GreatThis antibody works very well for western blot in MCF-7 cells
Date published: 2017-08-09
Rated 5 out of 5 by from The only choice you should make/If you need to detect hnRNP A1, look no further. This antibody provides great signal in all staining and blotting procedures. It has more than 40 citations to support it.
Date published: 2017-01-10
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hnRNP A1 抗体 (4B10) is rated 4.8 out of 5 by 18.
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