Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFκB family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Will this anti-HMGB2 antibody cross react with HMGB1 ?
Asked by: YKam
Thank you for your question. This monoclonal antibody is recommended to specifically detect HMG-2 (also designated HMGB2). However, we have not yet experimentally confirmed that it does not cross-react with HMG-1 (HMGB1).
We do offer an alternative monoclonal antibody, HMG-2 Antibody (D-3): sc-271689, which based on sequence analysis of the a short peptide antigen, is not expected to cross-react with HMG-1.
Answered by: Technical Support
Date published: 2020-07-14
For Western Blot, is it recommended to use denatured or non-denatured conditions with HMG-2 (68.32): sc-101067 antibody?
Asked by: Professor Griffin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.