HGK 抗体 (5-12): sc-100445

HGK Antibody (5-12) is an IgG₁ κ mouse monoclonal HGK antibody (also designated HGK antibody) that detects the HGK protein of human origin by WB, IP, IF, IHC(P) and ELISA. HGK Antibody (5-12) is available as the non-conjugated anti-HGK antibody. HGK (HPK/GC kinase-like kinase), also known as mitogen-activated protein kinase kinase kinase kinase 4, MAPK/ERK kinase kinase kinase 4, MEKKK 4 or NCK-interacting kinase (NIK), is a member of the serine/threonine kinase subfamily, Ste20. This subfamily is comprised of several mammalian kinases which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase, Ste20. Members of this subfamily include KHS, GLK, YSK1, HPK1, Krs-1, Krs-2, GC kinase and HGK. HGK, like many of the Ste20-like kinases, has been shown to activate the SAPK/JNK stress response pathway. HGK interacts with MEK kinase-1 and is thought to act upstream of MEK kinase-1 in the SAPK/JNK signaling pathway. Both the kinase domain and the C-terminal regulatory domain of HGK are required for full activation.

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References:

1. A novel GTP-binding protein hGBP3 interacts with NIK/HGK.  |  Luan, Z., et al. 2002. FEBS Lett. 530: 233-8. PMID: 12387898
2. The STE20 kinase HGK is broadly expressed in human tumor cells and can modulate cellular transformation, invasion, and adhesion.  |  Wright, JH., et al. 2003. Mol Cell Biol. 23: 2068-82. PMID: 12612079
3. The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components.  |  Leberer, E., et al. 1992. EMBO J. 11: 4815-24. PMID: 1464311
4. Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae.  |  Wu, C., et al. 1995. J Biol Chem. 270: 15984-92. PMID: 7608157
5. Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade.  |  Hu, MC., et al. 1996. Genes Dev. 10: 2251-64. PMID: 8824585
6. NIK is a new Ste20-related kinase that binds NCK and MEKK1 and activates the SAPK/JNK cascade via a conserved regulatory domain.  |  Su, YC., et al. 1997. EMBO J. 16: 1279-90. PMID: 9135144
7. Activation of the c-Jun N-terminal kinase pathway by a novel protein kinase related to human germinal center kinase.  |  Diener, K., et al. 1997. Proc Natl Acad Sci U S A. 94: 9687-92. PMID: 9275185
8. A novel human STE20-related protein kinase, HGK, that specifically activates the c-Jun N-terminal kinase signaling pathway.  |  Yao, Z., et al. 1999. J Biol Chem. 274: 2118-25. PMID: 9890973