Hck 抗体 (3D12E10): sc-101428

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Datasheets

- Hck 抗体 3D12E10 是小鼠单克隆 IgG₁ κ，Hck抗体, 在6篇文献中引用，规格为200 µg/ml
- 免疫human物种的重组Hck
- Hck 抗体 (3D12E10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Hck
- 抗-Hck 抗体 (3D12E10) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
- 还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
- 还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
- 关于如何获取Hck (3D12E10): sc-101428的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP and m-IgG₁ BP-HRP 是 Hck Antibody (3D12E10) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供Hck Antibody (3D12E10) (见下方订购信息).

SEE ALSO...

ChemCruz™ Chemical Hck Inhibitors可用于Hck细胞反应的功能性分析

Hck Antibody (3D12E10) - Western Blotting - Image 32803

Hck Antibody (3D12E10) - Western Blotting - Image 384637

Hck Antibody (3D12E10) - Western Blotting - Image 385931

Hck Antibody (3D12E10) - Western Blotting - Image 41118

Hck Antibody (3D12E10) - Western Blotting - Image 385930

Hck Antibody (3D12E10) - Western Blotting - Image 384638

Hck Antibody (3D12E10) - Western Blotting - Image 395725

Hck Antibody (3D12E10) - Western Blotting - Image 395896

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Hck Antibody (3D12E10) is an IgG₁ κ mouse monoclonal Hck antibody (also designated HCK Proto-Oncogene antibody, Src Family Tyrosine Kinase antibody, Tyrosine-Protein Kinase HCK antibody, Hematopoietic Cell Kinase antibody, P59-HCK/P60-HCK antibody, P59Hck antibody, P61Hck antibody, or JTK9 antibody) suitable for the detection of the Hck protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Hck Antibody (3D12E10) is available as both the non-conjugated anti-Hck antibody form, as well as multiple conjugated forms of anti-Hck antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Src is the human homolog of the v-src gene of the Rous sarcoma virus, also called avian sarcoma virus or ASV. Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. Different subcellular localizations of Src family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization and membrane trafficking. The human hemopoietic cell kinase (Hck) gene maps to chromosome 20q11-q12 and encodes a 505 amino acid protein. The Hck protein is expressed in hematopoietic cells, and is particularly abundant in granulocytes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	HCK	Entrez Gene ID: 3055	20q11.21	NM_001172129, NM_001172130, NM_001172131, NM_001172132, NM_001172133, NM_002110	UniProt ID: P08631	142370
Mouse	Hck	Entrez Gene ID: 15162	2 H1	NM_001172117, NM_010407	UniProt ID: P08103	N/A

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗Hck抗体(3D12E10)在各种科学出版物中有6篇引文。

针对哺乳动物靶标蛋白的一抗，如抗Hck 抗体 (3D12E10)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

Hck 抗体 (3D12E10) Specifications

替代产品名称

Hemopoietic Cell Kinase

产品克隆名称

3D12E10

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

抗原表位靶标

Hck

抗原表位种属

human

产品反应性/检测

Hck

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

6篇各种科学出版物的引文

可选的偶联物/形式

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase PE: Phycoerythrin

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗Hck抗体(3D12E10)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

Are there any issues with using sc-101428: Hck (3D12E10) mouse monoclonal primary antibody on mouse samples?

Asked by: AbPolly

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-101428 conjugates.

Answered by: Technical Support

Date published: 2017-03-07

Rated 4 out of 5 by LR from Band detected at expected mw in mouse Hck Band detected at expected mw in mouse Hck transfected 293T whole cell lysate. -SCBT QC

Date published: 2015-07-17

Rated 4 out of 5 by SH from Great Great, strong band in WB in U-937 and HL-60 whole cell lysates. -SCBT QC

Date published: 2015-05-13

Rated 4 out of 5 by MC from WB publishable data with human PBMCs and WB publishable data with human PBMCs and neutrophils - SCBT Publication Review

Date published: 2013-05-16

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