hCAP-H 抗体 (H-6): sc-101013

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- hCAP-H 抗体 H-6 是小鼠单克隆 IgG₁ κ，hCAP-H抗体, 在1篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组hCAP-H
- 推荐用于 human 来源的hCAP-H WB, IP 和 ELISA检测
- 关于如何获取hCAP-H (H-6): sc-101013的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成hCAP-H 抗体 (H-6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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此外 hCAP 抗体包括 hCAP-D2, hCAP-D3, hCAP-G 和 hCAP-H
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hCAP-H Antibody (H-6) - Western Blotting - Image 137032

hCAP-H Antibody (H-6) - Western Blotting - Image 52811

hCAP-H Antibody (H-6) - Western Blotting - Image 34377

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hCAP-H Antibody (H-6) is an IgG₁ κ mouse monoclonal hCAP-H antibody (also designated hCAP-H antibody) that detects the hCAP-H protein of human origin by WB, IP and ELISA. hCAP-H Antibody (H-6) is available as the non-conjugated anti-hCAP-H antibody. hCAP-H, also known as NCAPH (Non-SMC condensin I complex subunit H), BRRN, BRRN1 or CAPH, is a widely expressed 741 amino acid member of the CND2 (condensin subunit 2) family. Localized to the cytoplasm and the nucleus during interphase and to the nucleus during the rest of mitosis, hCAP-H is a regulatory subunit of the condensin complex, a multi-protein structure that converts interphase chromatin into condense chromosomes. The condensin complex is thought to induce positive supercoils into relaxed DNA and may also convert nicked DNA into knotted forms that can properly condense. hCAP-H, as well as other subunits of the condensin complex, are subject to phosphorylation by Cdc2 (cell division cycle 2). This phosphorylation activates the condensin complex and is, therefore, required for chromosome condensation.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

Chromosome condensation by a human condensin complex in Xenopus egg extracts. | Kimura, K., et al. 2001. J Biol Chem. 276: 5417-20. PMID: 11136719
Cell cycle-dependent expression and nucleolar localization of hCAP-H. | Cabello, OA., et al. 2001. Mol Biol Cell. 12: 3527-37. PMID: 11694586
Cnd2 has dual roles in mitotic condensation and interphase. | Aono, N., et al. 2002. Nature. 417: 197-202. PMID: 12000964
Condensin I interacts with the PARP-1-XRCC1 complex and functions in DNA single-strand break repair. | Heale, JT., et al. 2006. Mol Cell. 21: 837-48. PMID: 16543152
Phosphoproteome analysis of the human mitotic spindle. | Nousiainen, M., et al. 2006. Proc Natl Acad Sci U S A. 103: 5391-6. PMID: 16565220
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro. | Hirano, T. and Mitchison, TJ. 1994. Cell. 79: 449-58. PMID: 7954811
Chromatid segregation at anaphase requires the barren product, a novel chromosome-associated protein that interacts with Topoisomerase II. | Bhat, MA., et al. 1996. Cell. 87: 1103-14. PMID: 8978614
Localization of BRRN1, the human homologue of Drosophila barr, to 2q11.2. | Cabello, OA., et al. 1997. Genomics. 46: 311-3. PMID: 9417923

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	NCAPH	Entrez Gene ID: 23397	2q11.2	XM_005263908, XM_006712388, NM_001281710, NM_001281711, NM_001281712, NM_015341	UniProt ID: Q15003	602332

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗hCAP-H抗体(H-6)在各种科学出版物中有1篇引文。

针对哺乳动物靶标蛋白的一抗，如抗hCAP-H 抗体 (H-6)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

hCAP-H 抗体 (H-6) Specifications

替代产品名称

hCAP-H

产品克隆名称

H-6

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

645-741

抗原表位靶标

hCAP-H

抗原表位种属

human

产品反应性/检测

hCAP-H

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, ELISA

瓶的信息

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

1篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗hCAP-H抗体(H-6)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

For Western Blot, is it recommended to use denatured or non-denatured conditions with hCAP-H (H-6): sc-101013 antibody?

Asked by: DefinitelyNotMatt

Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.

Answered by: Technical Support

Date published: 2017-03-27

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hCAP-H 抗体 (H-6): sc-101013

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References:

hCAP-H 抗体 (H-6) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

For Western Blot, is it recommended to use denatured or non-denatured conditions with hCAP-H (H-6): sc-101013 antibody?