Date published: 2025-9-6
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H-Ras Antibody (259) - Western Blotting - Image 352077
H-Ras 抗体 (259): sc-35. K-562 (A), HeLa (B), Jurkat (C), NIH/3T3 (D), A-431 (E) 和 MCF7 (F) 全细胞裂解液中 H-Ras 表达的 Western 印迹分析.
H-Ras 抗体 (259): sc-35. K-562 (A), HeLa (B), Jurkat (C), NIH/3T3 (D), A-431 (E) 和 MCF7 (F) 全细胞裂解液中 H-Ras 表达的 Western 印迹分析.

H-Ras 抗体 (259): sc-35

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说明书
  • H-Ras 抗体 259 是大鼠单克隆 IgG1，H-Ras抗体, 在184篇文献中引用，规格为200 µg/ml
  • 抗-H-Ras 抗体 (259) 推荐用于 WB, IP, IF, FCM 和 ELISA，检测mouse, rat 和human 来源的 antigenic determinants common to H-, K- and N-Ras p21
  • H-Ras抗体（259）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于阻断H-Ras与Raf结合的无叠氮物（sc-35 L、200 µg/0.1 ml
  • 关于如何获取H-Ras (259): sc-35的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成H-Ras 抗体 (259)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Ras 抗体 包括 Ras-GRF1, κ B-Ras1, κ B-Ras2, E-Ras, H-Ras, N-Ras, R-Ras, pan Ras, Ras GAP和Ras2

H-Ras抗体(259)是一种IgG1大鼠单克隆H-Ras抗体(也称为HRAS抗体)，通过WB、IP、IF、FCM和ELISA检测小鼠、大鼠和人来源的H-Ras蛋白。H-Ras抗体(259)既可以是非偶联的抗H-Ras抗体形式，也可以是多种偶联形式的抗H-Ras抗体，包括琼脂、HRP、PE、FITC和多种Alexa Fluor®偶联物。哺乳动物Ras（也称为v-Ha-Ras、Harvey大鼠肉瘤病毒致癌基因同源物、HRAS1、K-Ras、N-Ras、RASH1或c-bas/has）基因家族由Harvey和Kirsten Ras基因（c-H-Ras1和c-K-Ras2）、每个基因的一个无活性假基因（c-H-Ras2和c-K-Ras1）以及N-Ras基因组成。三种致癌Ras，H-Ras、K-Ras和N-Ras，编码具有GTP/GDP结合和GTP酶作用的蛋白质。Ras蛋白在GDP结合的无活性形式和GTP结合的活性形式之间交替，由一种Guanine核苷交换因子（GEF）激活，由一种GTP酶激活蛋白（GAP）失活。Ras命名源于对人类DNA序列的描述，这些序列与含有哺乳动物C型逆转录病毒（Harvey株小鼠肉瘤病毒，来自大鼠）致癌序列的克隆DNA片段同源。在正常情况下，Ras家族成员影响细胞生长和分化事件的一个基于亚细胞膜分组信号系统。致癌Ras可以调节控制细胞增殖和凋亡的过程。GTP水解耦合信号转导中继蛋白Ras超家族可以分为Ras、Rho、Rab和ARF家族。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

H-Ras 抗体 (259) 参考文献:

  1. H-ras 依靠 GTP 从脂质筏中分离是生物活性的必要条件。  |  Prior, IA., et al. 2001. Nat Cell Biol. 3: 368-75. PMID: 11283610
  2. H-Ras信号和K-Ras信号对内吞作用的依赖性不同。  |  Roy, S., et al. 2002. Mol Cell Biol. 22: 5128-40. PMID: 12077341
  3. RasGRF 家族鸟嘌呤核苷酸交换因子激活内质网中的 H-Ras。  |  Arozarena, I., et al. 2004. Mol Cell Biol. 24: 1516-30. PMID: 14749369
  4. 三个可分离的域调节H-ras与质膜的GTP依赖性结合。  |  Rotblat, B., et al. 2004. Mol Cell Biol. 24: 6799-810. PMID: 15254246
  5. 在H-ras的运输、微定位和信号传导中，单个棕榈酰基残基起着不同的作用。  |  Roy, S., et al. 2005. Mol Cell Biol. 25: 6722-33. PMID: 16024806
  6. H-Ras与CHO-K1细胞中的再循环内体动态相互作用：Rab5和Rab11参与H-Ras向该细胞质中心内吞腔的运输。  |  Gomez, GA. and Daniotti, JL. 2005. J Biol Chem. 280: 34997-5010. PMID: 16079139
  7. 一种新型开关区域调节H-ras的膜定向和信号输出。  |  Abankwa, D., et al. 2008. EMBO J. 27: 727-35. PMID: 18273062
  8. 非甾体类抗炎药改变质膜上Ras蛋白的空间时间组织。  |  Zhou, Y., et al. 2012. J Biol Chem. 287: 16586-95. PMID: 22433858
  9. H-ras在质膜微域中的分布和信号传导受酰化和脱酰化事件的调控。  |  Agudo-Ibáñez, L., et al. 2015. Mol Cell Biol. 35: 1898-914. PMID: 25776558
  10. 单个S-酰化半胱氨酸对H-Ras内质膜转运和酰化/脱酰化循环有不同影响。  |  Pedro, MP., et al. 2017. Mol Biol Cell. 28: 962-974. PMID: 28179458

订购信息

产品名称产品编号规格价格数量收藏夹

H-Ras 抗体 (259)

sc-35
200 µg/ml
RMB2377.00

H-Ras 抗体 (259) L

sc-35 L
200 µg/0.1 ml
RMB2377.00

H-Ras 抗体 (259) AC

sc-35 AC
500 µg/ml, 25% agarose
RMB3129.00

H-Ras 抗体 (259) HRP

sc-35 HRP
200 µg/ml
RMB2377.00

H-Ras 抗体 (259) FITC

sc-35 FITC
200 µg/ml
RMB2482.00

H-Ras 抗体 (259) PE

sc-35 PE
200 µg/ml
RMB2580.00

H-Ras 抗体 (259) Alexa Fluor® 488

sc-35 AF488
200 µg/ml
RMB2685.00

H-Ras 抗体 (259) Alexa Fluor® 546

sc-35 AF546
200 µg/ml
RMB2685.00

H-Ras 抗体 (259) Alexa Fluor® 594

sc-35 AF594
200 µg/ml
RMB2685.00

H-Ras 抗体 (259) Alexa Fluor® 647

sc-35 AF647
200 µg/ml
RMB2685.00

H-Ras 抗体 (259) Alexa Fluor® 680

sc-35 AF680
200 µg/ml
RMB2685.00

H-Ras 抗体 (259) Alexa Fluor® 790

sc-35 AF790
200 µg/ml
RMB2685.00

H-Ras (259) 中和勝肽

sc-35 P
100 µg/0.5 ml
RMB511.00

What is the incubation time and tempreture of sc-35 PE for flow cytometry? I tried 1ug consentration for 1 million cells at room tempreture, 30 min but it didn't work.

Asked by: Burcu
Thank you for your question. Please contact your local distributor or technical service directly for assistance regarding your FCM results, we are happy to help troubleshoot this with you. We recommend a incubation time of ~30 minutes on ice. You can find the recommended protocol here: https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: Tech Service
Date published: 2019-11-15

Según el datasheet aunque pone es monoclonal de H-Ras reconoce también a N- y K-Ras ¿es correcto?

Asked by: Arancha
Gracias por preguntar. Es correcto, H-Ras (259): sc-35 es recomendado para detectar H-Ras, K-Ras y N-Ras. Thank you for your question. Yes, H-Ras (259): sc-35 is recommended for detecting H-Ras, K-Ras and N-Ras.
Answered by: Technical Support
Date published: 2018-12-06

"for detection of antigenic determinants common to H-, K- and N-Ras" The antibody is labeled as an H-ras antibody but the description says it will detect H-, K-, N-ras. Am I understanding the description wrong? We want an IgG specific for H-ras

Asked by: Quan2017
Thank you for your question. You are correct, the mouse monoclonal, H-Ras (259): sc-35, antibody has an epitope which is highly conserved in the Ras protein family. This clone is extremely popular, as indicated by the 75 product citations. However, if you are looking for a primary antibody that is specific for H-Ras then we would recommend one of our alternative clones: sc-29, sc-53958 or sc-53959. The most popular among these antibodies is our H-Ras Antibody (F235): sc-29 (https://www.scbt.com/scbt/product/h-ras-antibody-f235).
Answered by: Technical Support
Date published: 2017-01-26

I am in need of a new secondary reagent. Do you have any suggestions for what works best with sc-35?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-18

What is the difference between sc-35 and sc-35 P?

Asked by: AbPolly
Thank you for your inquiry. sc-35 is our standard unconjugated H-Ras (259) monoclonal antibody. sc-35 P is the blocking peptide matching this antibody that can be used as a negative control in combination with the antibody. For the protocol on how to use the blocking peptide, please see here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Service
Date published: 2016-12-23
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Rated 5 out of 5 by from Great pull downGot nice signal of activated HRas after pull down.
Date published: 2024-05-30
Rated 5 out of 5 by from Early and Late Induction of KRAS and HRASEarly and Late Induction of KRAS and HRAS Proto-Oncogenes by Reactive Oxygen Species in Primary Astrocytes
Date published: 2020-10-16
Rated 4 out of 5 by from Works well in WBI got the sample afew days ago ,and It is work well in WB at a 1:200 difution
Date published: 2017-04-24
Rated 1 out of 5 by from did not workOverexpressed GFP-H-ras (human clone) in 293 cells was not detected. there is no band in 293 cells. There are no non-specific band. 1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-03-23
Rated 5 out of 5 by from Superb !!Clean western blot data with strong signal and low background; also grea IF staining data
Date published: 2017-02-01
Rated 5 out of 5 by from Good antibody for flowVery strong, clear signal. Well-cited in a variety of applications including flow cytometry and ELISA.
Date published: 2016-12-29
Rated 5 out of 5 by from Worked well in IP with Jurkat lysatesWorked well in IP with Jurkat lysates -SCBT Publication Review
Date published: 2015-04-17
Rated 4 out of 5 by from GoodGood, specific staining of cytoplasm with methanol-fixed HeLa cells in IF -SCBT QC
Date published: 2015-03-09
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H-Ras 抗体 (259) is rated 4.5 out of 5 by 11.
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