GFP抗体 (15) 推荐用于 GFP IF 和 IHC(P)检测

GFP 抗体 (15): sc-101525

GFP抗体 (15) is rated 4.8 out of 5 by 12.
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Datasheets

    • GFP抗体(15)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
    • raised against recombinant GFP
    • 推荐用于 GFP IF 和 IHC(P)检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)

You recommended the GFP Antibody (15): sc-101525 for detection of GFP by IHC(P) but I see no picture and cannot find any published data? Could you share your data or help me find published papers. I am OK to test a free sample of the antibody. Thx

Asked by: UCRes
Thank you for your question. We recommend this mouse monoclonal antibody, GFP (15): sc-101525, for detection of GFP by IF and IHC(P) based on publications in the literature. For one reference, please refer to the publication below on PubMed, PMID: 20099318, which is also listed as a product citation on our website (with a link out to PubMed). We are not currently offering a free sample size for this particular antibody. As an alternative, if you would like to test a free sample of GFP Antibody (B-2): sc-9996, please contact our Technical Service Department. This antibody is not currently recommended for IHC(P) because we have not yet tested the antibody with FFPE, however, it also has been published for this application and may be suitable.
Answered by: Technical Support
Date published: 2017-09-11

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with GFP Antibody (15): sc-101525?

Asked by: Trav11
Thank you for your question. We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 3
Date published: 2017-02-03
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Rated 5 out of 5 by from Great antibody Worked very well with Western blotting and IP. Would highly recommend this antibody.
Date published: 2019-05-10
Rated 4 out of 5 by from GOOD I had this one to test the CONSTRUCT, and it worked well in HEK lysate
Date published: 2018-05-14
Rated 5 out of 5 by from Really good antibody Got this as sample.Now ordering it for future use.
Date published: 2017-12-16
Rated 5 out of 5 by from Good effect GFP used for detect tumor cell, IF of this antibody is obvious.
Date published: 2017-09-06
Rated 5 out of 5 by from Good Antibody! We used this antibody for IF staining and WB, and it has got great results with a nice clear image.
Date published: 2017-09-04
Rated 3 out of 5 by from One work, one didn't first one I bought didn't work. Then the tech person sent me two more, same one I picked still not work. the other one he suggested worked.
Date published: 2017-03-23
Rated 5 out of 5 by from Great IF stain We used this antibody for IF staining and got great results with a nice clear image
Date published: 2017-01-10
Rated 5 out of 5 by from This antibody is good for Western Blot I used GFP-tag antibody at a dilution of 1:2000 in PBST for 1 h and is enough for an excellent Western blot signal when the protein from DF-1 cell lysate.
Date published: 2016-11-24
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