GC-C 抗体 (537): sc-100302

4.8(4)

写一个评价提问

Datasheets

- GC-C 抗体 537 是小鼠单克隆 IgG_2b κ，GC-C抗体, 在3篇文献中引用，规格为100 µg/ml
- raised against partial recombinant protein mapping at the N-terminus of GC-C of human origin
- 推荐用于 human 来源的GC-C WB, IP 和 ELISA检测
- 关于如何获取GC-C (537): sc-100302的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG_2b BP-HRP 是GC-C Antibody (537) for WB首选应用二抗. 这些试剂现在以套装形式提供GC-C Antibody (537) (见下方订购信息).

SEE ALSO...

ChemCruz™ Chemical Guanylate Cyclases Inhibitors or Guanylate Cyclases Activators可用于Guanylate Cyclases细胞反应的功能性分析

GC-C Antibody (537) - Western Blotting - Image 33676

点击图片或放大按钮来放大

GC-C Antibody (537) is an IgG_2b κ mouse monoclonal GC-C antibody (also designated GC-C antibody) that detects the GC-C protein of human origin by WB, IP and ELISA. GC-C Antibody (537) is available as the non-conjugated anti-GC-C antibody. Guanylate Cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase, a soluble form (GCS or sGC), which act as receptors for nitric oxide, and a membrane-bound receptor form (GC), which are peptide hormone receptors. The GC-C protein is composed of an extracellular domain, a single transmembrane domain, and a cytoplasmic region consisting of a kinase-like domain and a catalytic domain. It is expressed as two differentially glycosylated forms, a precursor form present in the endoplasmic reticulum and a form present on the plasma membrane. Ligand binding to the extracellular domain of GC-C promotes the accumulation of cGMP. GC-C acts as the receptor for heat-stable enterotoxins, small peptides secreted by some pathogenic strains of E. coli that cause severe secretory diarrhea. GC-C also binds to guanylin and uroguanylin peptides, which modulate renal function in response to oral salt load.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

Guanylate cyclase and the .NO/cGMP signaling pathway. | Denninger, JW. and Marletta, MA. 1999. Biochim Biophys Acta. 1411: 334-50. PMID: 10320667
In vivo control of soluble guanylate cyclase activation by nitric oxide: a kinetic analysis. | Condorelli, P. and George, SC. 2001. Biophys J. 80: 2110-9. PMID: 11325714
Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C. | Ghanekar, Y., et al. 2003. Eur J Biochem. 270: 3848-57. PMID: 12950269
Glycosylation of the receptor guanylate cyclase C: role in ligand binding and catalytic activity. | Ghanekar, Y., et al. 2004. Biochem J. 379: 653-63. PMID: 14748740
Cardiac and intestinal natriuretic peptides: insights from genetically modified mice. | Kuhn, M. 2005. Peptides. 26: 1078-85. PMID: 15911075
Enterotoxin/guanylin receptor type guanylyl cyclases in non-mammalian vertebrates. | Nakauchi, M. and Suzuki, N. 2005. Zoolog Sci. 22: 501-9. PMID: 15930822

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	GUCY2C	Entrez Gene ID: 2984	12p13.1	XM_011520631, NM_004963	UniProt ID: P25092	601330

Download Datasheet

Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗GC-C抗体(537)在各种科学出版物中有3篇引文。

针对哺乳动物靶标蛋白的一抗，如抗GC-C 抗体 (537)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

GC-C 抗体 (537) Specifications

替代产品名称

GC-C

产品克隆名称

537

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG_2b kappa light chain

氨基酸

24-133

抗原表位靶标

GC-C

抗原表位种属

human

产品反应性/检测

GC-C

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, ELISA

瓶的信息

Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

3篇各种科学出版物的引文

可选的偶联物/形式

N/A

果蝇

非洲爪蟾蜍

拟南芥

秀丽隐杆线虫

梭菌

弯曲杆菌

幽门螺杆菌

肝炎

克雷白氏杆菌

结核分支杆菌

疟原虫

假单胞菌

酿酒酵母

沙门氏菌

血吸虫

志贺氏杆菌

葡萄球菌

How much protein do you recommend loading on the gel for Western blot detection using GC-C (537): sc-100302?

Asked by: jenni

We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting

Answered by: Technical Support 3

Date published: 2017-02-08

Rated 5 out of 5 by BMMcGill from Good for WB in mice We used GC-C (537) antibody for WB in Mouse lung primary fibroblast, and it worked very well. We used anti-mouse IgG.

Date published: 2018-03-28

Rated 5 out of 5 by cjMara from Good for WB! This monoclonal GC-C Antibody (537) is a good choice for Western blotting applications in human samples.

Date published: 2017-01-27

Rated 5 out of 5 by E_R_ from T84 colon cancer cell line T84 colon cancer cell line, the staining detected with Flow Citometry was fine

Date published: 2015-09-07

选择店铺

选择语言

选择币种

GC-C 抗体 (537): sc-100302

QUICK LINKS

SEE ALSO...

References:

GC-C 抗体 (537) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

How much protein do you recommend loading on the gel for Western blot detection using GC-C (537): sc-100302?