GC-C抗体 (537) 推荐用于 human 来源的GC-C WB, IP 和 ELISA检测

GC-C 抗体 (537): sc-100302

GC-C抗体 (537) is rated 4.8 out of 5 by 4.
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Datasheets

    • 抗-GC-C 抗体 537 是小鼠单克隆 IgG2b (kappa轻链),GC-C抗体,规格为100 µg/ml
    • raised against partial recombinant protein mapping at the N-terminus of GC-C of human origin
    • 推荐用于 human 来源的GC-C WB, IP 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取GC-C (537): sc-100302免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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How much protein do you recommend loading on the gel for Western blot detection using GC-C (537): sc-100302?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 3
Date published: 2017-02-08
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Rated 5 out of 5 by from Good for WB in mice We used GC-C (537) antibody for WB in Mouse lung primary fibroblast, and it worked very well. We used anti-mouse IgG.
Date published: 2018-03-28
Rated 5 out of 5 by from Good for WB! This monoclonal GC-C Antibody (537) is a good choice for Western blotting applications in human samples.
Date published: 2017-01-27
Rated 5 out of 5 by from T84 colon cancer cell line T84 colon cancer cell line, the staining detected with Flow Citometry was fine
Date published: 2015-09-07
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