GATAD2B 抗体 (ZZ-9): sc-101052

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Datasheets

- GATAD2B 抗体 ZZ-9 是小鼠单克隆 IgG_2a (kappa轻链)，GATAD2B抗体，规格为100 µg/ml
- 免疫human物种的重组GATAD2B
- 推荐用于 mouse, rat 和 human 来源的GATAD2B WB, IP 和 ELISA检测
- 关于如何获取GATAD2B (ZZ-9): sc-101052的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成GATAD2B 抗体 (ZZ-9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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此外 GATAD 抗体包括 GATAD1, GATAD2A和 GATAD2B
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

GATAD2B Antibody (ZZ-9) - Western Blotting - Image 34419

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GATAD2B Antibody (ZZ-9) is an IgG_2a κ mouse monoclonal GATAD2B antibody (also designated GATAD2B antibody) that detects the GATAD2B protein of mouse, rat and human origin by WB, IP and ELISA. GATAD2B Antibody (ZZ-9) is available as the non-conjugated anti-GATAD2B antibody. GATAD2B (GATA zinc finger domain containing 2B), also known as P66beta, is a 593 amino acid protein that contains one GATA-type zinc finger and localizes to discrete foci within the nucleus. Expressed ubiquitously, GATAD2B is thought to function as a transcriptional repressor that interacts with the methyl-CpG-binding protein MBD3, a component of the MeCP1 complex. MeCP1 is a multi-subunit complex that represses transcription via histone deacetylation and nucleosomal remodeling, thereby condensing chromatin structure and preventing transcription. GATAD2B interacts with and recruits MBD3 to specific areas within the nucleus, thereby participating in MeCP1 complex-mediated transcriptional repression. GATAD2B is expressed in various cancer cell lines, suggesting a possible role in carcinogenesis.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

Identification and functional characterization of the p66/p68 components of the MeCP1 complex. | Feng, Q., et al. 2002. Mol Cell Biol. 22: 536-46. PMID: 11756549
Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3. | Brackertz, M., et al. 2002. J Biol Chem. 277: 40958-66. PMID: 12183469
p66alpha and p66beta of the Mi-2/NuRD complex mediate MBD2 and histone interaction. | Brackertz, M., et al. 2006. Nucleic Acids Res. 34: 397-406. PMID: 16415179
SUMO modification enhances p66-mediated transcriptional repression of the Mi-2/NuRD complex. | Gong, Z., et al. 2006. Mol Cell Biol. 26: 4519-28. PMID: 16738318
Mutants in the mouse NuRD/Mi2 component P66alpha are embryonic lethal. | Marino, S. and Nusse, R. 2007. PLoS One. 2: e519. PMID: 17565372

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	GATAD2B	Entrez Gene ID: 57459	1q21.3	XM_005245364, NM_020699	UniProt ID: Q8WXI9	n/a
Mouse	Gatad2b	Entrez Gene ID: 229542	3 F1	XM_006501359, XM_006501360, XM_006501361, NM_139304	UniProt ID: Q8VHR5	N/A

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针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗GATAD2B抗体(ZZ-9)在各种科学出版物中有0篇引文。

针对哺乳动物靶标蛋白的一抗，如抗GATAD2B 抗体 (ZZ-9)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

GATAD2B 抗体 (ZZ-9) Specifications

替代产品名称

GATAD2B

产品克隆名称

ZZ-9

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG_2a kappa light chain

氨基酸

3-111

抗原表位靶标

GATAD2B

抗原表位种属

human

产品反应性/检测

GATAD2B

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, ELISA

瓶的信息

Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

0篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗GATAD2B抗体(ZZ-9)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

这个抗体的识别位点在哪里？

Asked by: 小太阳hao

Thank you for your question. GATAD2B (ZZ-9): sc-101052 is raised against amino acids 3-111 of human recombinant GATAD2B protein. 感谢您的提问。GATAD2B 抗体 (ZZ-9): sc-101052是针对人源重组蛋白GATAD2B的3-111个氨基酸作为抗原生产的抗体.

Answered by: Technical Support 9

Date published: 2019-12-04

For Western Blot, is it recommended to use denatured or non-denatured conditions with GATAD2B (ZZ-9): sc-101052 antibody?

Asked by: Trav11

Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.

Answered by: Technical Support

Date published: 2017-03-27

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GATAD2B 抗体 (ZZ-9): sc-101052

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References:

GATAD2B 抗体 (ZZ-9) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

这个抗体的识别位点在哪里？

For Western Blot, is it recommended to use denatured or non-denatured conditions with GATAD2B (ZZ-9): sc-101052 antibody?