gankyrin抗体 (3A6C2) 推荐用于 mouse, rat 和 human 来源的gankyrin WB, IP 和 IF检测

gankyrin 抗体 (3A6C2): sc-101498

gankyrin抗体 (3A6C2) is rated 5.0 out of 5 by 2.
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Datasheets

    • 抗-gankyrin 抗体 3A6C2 是小鼠单克隆 IgG2b (kappa轻链),gankyrin抗体,规格为200 µg/ml
    • 免疫human物种的重组gankyrin
    • 抗-gankyrin 抗体 (3A6C2) 推荐用于 WB, IP 和 IF,检测mouse, rat 和human 来源的 gankyrin
    • 抗-gankyrin 抗体 (3A6C2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取gankyrin (3A6C2): sc-101498免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Hi, What is the immunogen used to produce this antibody or in other words, which region on the recombinant gankyrin protein does this antibody bind to? Thanks

Asked by: Abbas
Thank you for your question. This mouse monoclonal gankyrin Antibody (3A6C2) was raised against full length recombinant human gankyrin. According to the following publication, the epitope mapping is somewhere between "the third and fifth ankyrin‐repeat region", or between amino acids 72-170. https://aasldpubs.onlinelibrary.wiley.com/doi/full/10.1002/hep.22027
Answered by: Technical Support
Date published: 2019-01-04

For Western Blot, is it recommended to use denatured or non-denatured conditions with gankyrin (3A6C2): sc-101498 monoclonal antibody?

Asked by: TinTin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from 免疫荧光效果很好 用来做免疫荧光实验,效果很好,细胞定位很清晰,图片效果也很好,会继续使用,Western实验还没有做,下次继续
Date published: 2018-01-05
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