FUT2 抗体 (9T-8): sc-100742

FUT2 Antibody (9T-8) is an IgG_2a κ mouse monoclonal FUT2 antibody (also designated FUT2 antibody) suitable for the detection of the FUT2 protein of mouse, rat and human origin by WB, IP and ELISA. FUT2 Antibody (9T-8) is available as the non-conjugated anti-FUT2 antibody. All human blood, with rare exception, carries the red cell H antigen. The H blood group locus determines expression of the H antigen in the erythroid lineage, whereas a unique locus (the SE (secretion) locus) controls H expression in a variety of secretory epithelia and in saliva. Individuals of the Bombay phenotype lack H antigen, whereas individuals of the para-Bombay phenotype synthesize H determinants (essential precursors to A and B antigens) in their secretory epithelia but not in the erythroid lineage (2-4). The H and SE loci, which may have arisen by gene duplication from a common ancestral gene, are known as FUT1 and FUT2, respectively, and are tightly linked on chromosome 19q13.3. FUT1 and FUT2 encode two distinct a-2-L-fucosyltransferases in human serum. The FUT2 locus (SE or ABO-Secretor locus) exhibits extensive polymorphism showing high heterogeneity and overt ethnic specificity. For this reason, mutations or polymorphisms of the FUT2 gene are used as markers for investigating population genetics. FUT2 is expressed on the surface of several human tumor cell lines such as BEL-7404, SPC-A-1, and SGC-7901 (10).

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References:

1. Molecular cloning, genomic mapping, and expression of two secretor blood group alpha (1,2)fucosyltransferase genes differentially regulated in mouse uterine epithelium and gastrointestinal tract.  |  Domino, SE., et al. 2001. J Biol Chem. 276: 23748-56. PMID: 11323419
2. FUT2 gene involved in expression of H blood group antigen on surface of human tumor cell lines BEL-7404, SGC-7901, and SPC-A-1.  |  Xing, L. and Guo, LH. 2000. Acta Pharmacol Sin. 21: 997-1001. PMID: 11501068
3. Organization of the bovine alpha 2-fucosyltransferase gene cluster suggests that the Sec1 gene might have been shaped through a nonautonomous L1-retrotransposition event within the same locus.  |  Saunier, K., et al. 2001. Mol Biol Evol. 18: 2083-91. PMID: 11606704
4. Polymorphism and distribution of the Secretor alpha(1,2)-fucosyltransferase gene in various Taiwanese populations.  |  Yu, LC., et al. 2001. Transfusion. 41: 1279-84. PMID: 11606829
5. Polymorphism of the human ABO-Secretor locus (FUT2) in four populations in Asia: indication of distinct Asian subpopulations.  |  Pang, H., et al. 2001. Ann Hum Genet. 65: 429-37. PMID: 11806852
6. Molecular analysis of mutations and polymorphisms of the Lewis secretor type alpha(1,2)-fucosyltransferase gene reveals that Taiwan aborigines are of Austronesian derivation.  |  Chang, JG., et al. 2002. J Hum Genet. 47: 60-5. PMID: 11916003
7. Molecular basis for H blood group deficiency in Bombay (Oh) and para-Bombay individuals.  |  Kelly, RJ., et al. 1994. Proc Natl Acad Sci U S A. 91: 5843-7. PMID: 7912436
8. Two missense mutations of H type alpha(1,2)fucosyltransferase gene (FUT1) responsible for para-Bombay phenotype.  |  Wang, B., et al. 1997. Vox Sang. 72: 31-5. PMID: 9031498
9. Missense mutation of FUT1 and deletion of FUT2 are responsible for Indian Bombay phenotype of ABO blood group system.  |  Koda, Y., et al. 1997. Biochem Biophys Res Commun. 238: 21-5. PMID: 9299444