FNDC3A 抗体 (J-7): sc-100771

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- FNDC3A 抗体 J-7 是小鼠单克隆 IgG_2b (kappa轻链)，FNDC3A抗体，规格为100 µg/ml
- 免疫human物种的重组FNDC3A
- 推荐用于 human 来源的FNDC3A WB, IP, IF 和 ELISA检测
- 目前，我们还没有完成FNDC3A 抗体 (J-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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此外 FNDC 抗体包括 FNDC3B
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FNDC3A Antibody (J-7) - Immunofluorescence - Image 35669

FNDC3A Antibody (J-7) - Western Blotting - Image 34144

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FNDC3A Antibody (J-7) is an IgG_2b κ mouse monoclonal FNDC3A antibody (also designated FNDC3A antibody) that detects the FNDC3A protein of human origin by WB, IP, IF and ELISA. FNDC3A Antibody (J-7) is available as the non-conjugated anti-FNDC3A antibody. FNDC3A (fibronectin type III domain containing 3A), also known as HUGO (human gene expressed in odontoblasts), is a 1,134 amino acid protein that belongs to the FNDC3 family of proteins. FNDC3A contains an N-terminal proline-rich region, nine fibronectin type-III domains (none of which contain an RGD sequence) and a hydrophobic C-terminal transmembranous helix. Expressed in a wide variety of tissues, FNDC3A localizes to Golgi vesicles and to the developing acrosome of spermatids. FNDC3A is believed to function in glycosaminoglycan and collagen synthesis. In mice, a mutation in the gene encoding FNDC3A causes male sterility due to defective adhesion between Sertoli cells and spermatids in the seminiferous epithelium. This suggests that FNDC3A plays an important role in spermatogenesis, possibly mediating or maintaining the adhesion between Sertoli cells and spermatids.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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References:

Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. | Nagase, T., et al. 1999. DNA Res. 6: 63-70. PMID: 10231032
Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. | Nakajima, D., et al. 2002. DNA Res. 9: 99-106. PMID: 12168954
FNDC3A is required for adhesion between spermatids and Sertoli cells. | Obholz, KL., et al. 2006. Dev Biol. 298: 498-513. PMID: 16904100
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. | Olsen, JV., et al. 2006. Cell. 127: 635-48. PMID: 17081983
HUGO (FNDC3A): a new gene overexpressed in human odontoblasts. | Carrouel, F., et al. 2008. J Dent Res. 87: 131-6. PMID: 18218838
Normalization and subtraction: two approaches to facilitate gene discovery. | Bonaldo, MF., et al. 1996. Genome Res. 6: 791-806. PMID: 8889548

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	FNDC3A	Entrez Gene ID: 22862	13q14.2	XM_011534997, XM_017020440, XM_017020441, XM_017020442, XM_017020443, NM_001079673, NM_001278438, NM_014923	UniProt ID: Q9Y2H6	n/a

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I am using FNDC3A (J-7): sc-100771 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Germaine

Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 4 out of 5 by HC from Clear immunofluorescence membrane and cytoplasmic Clear immunofluorescence membrane and cytoplasmic staining in paraformaldehyde-fixed A-431 cells. -SCBT QC

Date published: 2015-02-03

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FNDC3A 抗体 (J-7): sc-100771

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I am using FNDC3A (J-7): sc-100771 for immunofluorescence, what dilution of this antibody should I use?