FLJ23356 抗体 (S-23): sc-100433

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- FLJ23356 抗体 S-23 是小鼠单克隆 IgG₁ κ，FLJ23356抗体, 在4篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组FLJ23356
- 推荐用于 human 来源的FLJ23356 WB, IP, IF 和 ELISA检测
- 关于如何获取FLJ23356 (S-23): sc-100433的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成FLJ23356 抗体 (S-23)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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FLJ23356 Antibody (S-23) - Western Blotting - Image 33806

FLJ23356 Antibody (S-23) - Immunofluorescence - Image 35565

FLJ23356 Antibody (S-23) - Western Blotting - Image 158358

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FLJ23356 Antibody (S-23) is an IgG₁ κ mouse monoclonal FLJ23356 antibody (also designated FLJ23356 antibody) suitable for the detection of the FLJ23356 protein of human origin by WB, IP, IF and ELISA. FLJ23356 Antibody (S-23) is available as the non-conjugated anti-FLJ23356 antibody. Serine/Threonine protein kinases possess a catalytic subunit which transfers the gamma phosphate from nucleotide triphosphates to one or more amino acid residue in a protein substrate side chain, resulting in a conformational change that affects protein function. Serine/Threonine kinases play a role in various cellular processes, including division, proliferation, differentiation and apoptosis. The catalytic subunits of Serine/Threonine kinases are highly conserved between species. FLJ23356, also known as Sugen kinase 196, SGK196 or protein kinase-like protein SgK196, is a 350 amino acid protein that belongs to the Serine/Threonine protein kinase family. FLJ23356 is thought to have a kinase domain that is catalytically inactive. It has been suggested that FLJ23356 may have a glycine-to-serine substitution motif at subdomain VII of its catalytic domain.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

Evolution of protein kinase signaling from yeast to man. | Manning, G., et al. 2002. Trends Biochem Sci. 27: 514-20. PMID: 12368087
High-throughput structural biology in drug discovery: protein kinases. | Stout, TJ., et al. 2004. Curr Pharm Des. 10: 1069-82. PMID: 15078142
Creating chemical diversity to target protein kinases. | Li, B., et al. 2004. Comb Chem High Throughput Screen. 7: 453-72. PMID: 15320712
LRRK2 phosphorylates moesin at threonine-558: characterization of how Parkinson′s disease mutants affect kinase activity. | Jaleel, M., et al. 2007. Biochem J. 405: 307-17. PMID: 17447891
Protein kinase catalytic domain sequence database: identification of conserved features of primary structure and classification of family members. | Hanks, SK. and Quinn, AM. 1991. Methods Enzymol. 200: 38-62. PMID: 1956325
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. | Hanks, SK., et al. 1988. Science. 241: 42-52. PMID: 3291115
Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification. | Hanks, SK. and Hunter, T. 1995. FASEB J. 9: 576-96. PMID: 7768349

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	POMK	Entrez Gene ID: 84197	8p11.21	NM_001277971, NM_032237	UniProt ID: Q9H5K3	n/a

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗FLJ23356抗体(S-23)在各种科学出版物中有4篇引文。

针对哺乳动物靶标蛋白的一抗，如抗FLJ23356 抗体 (S-23)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

FLJ23356 抗体 (S-23) Specifications

替代产品名称

FLJ23356

产品克隆名称

S-23

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

251-351

抗原表位靶标

FLJ23356

抗原表位种属

human

产品反应性/检测

FLJ23356

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, ELISA

瓶的信息

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

4篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗FLJ23356抗体(S-23)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

I am using FLJ23356 (S-23): sc-100433 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Dr Ninau Qelp

Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 5 out of 5 by ki48 from Detects endogenous protein in Western blot Antibody works well in Western blotting and detects endogenous protein in cell lysates (1:1000, BSA blocking).

Date published: 2022-08-27

Rated 5 out of 5 by TO from Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC

Date published: 2015-02-01

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FLJ23356 抗体 (S-23): sc-100433

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References:

FLJ23356 抗体 (S-23) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

I am using FLJ23356 (S-23): sc-100433 for immunofluorescence, what dilution of this antibody should I use?