Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
FHL-5 Antibody (F-26) is a high quality monoclonal FHL-5 antibody (also designated FHL-5 antibody) suitable for the detection of the FHL-5 protein of human origin. FHL-5 Antibody (F-26) is available as the non-conjugated anti-FHL-5 antibody. The four-and-a-half-LIM domain (FHL) proteins include FHL-1 (SLIM1), FHL-2 (SLIM3), FHL-3 (SLIM2), FHL-4 and FHL-5. The signature "half-domain", a single zinc finger domain located in the N-terminal region, differentiates FHLs from other LIM-only proteins, which have numbers of zinc fingers. Specific combinations of FHL proteins elicit selective activation of both CREB and CREM. FHL-5, also known as ACT (Activator of CREM in Testis), is a testis-specific protein that interacts with CREM (a transcription factor required for spermatid differentiation) via its third LIM domain and can stimulate CREM activity independently of phosphorylation. This suggests that FHL-5 may participate in the regulation of spermatogenesis by acting as a transcriptional coactivator of CREM. During spermatid elongation, FHL-5 is translocated from the nucleus to the cytoplasm by the kinesin motor protein KIF17 thus silencing CREM activity.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using FHL-5 (F-26): sc-101045 for immunofluorescence, what dilution of this antibody should I use?
Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: