FBXO42抗体 (FL-6) 推荐用于 mouse, rat 和 human 来源的FBXO42 WB, IP 和 ELISA检测

FBXO42 抗体 (FL-6): sc-100737

FBXO42抗体 (FL-6) is rated 4.3 out of 5 by 4.
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Datasheets

    • 抗-FBXO42 抗体 FL-6 是小鼠单克隆 IgG2a (kappa轻链),FBXO42抗体,规格为100 µg/ml
    • 免疫human物种的重组FBXO42
    • 推荐用于 mouse, rat 和 human 来源的FBXO42 WB, IP 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取FBXO42 (FL-6): sc-100737免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Did anybody use this antibody for immunoprecipitation? What was the concencentraion used?

Asked by: mujib
Thank you for your question. This mouse monoclonal antibody, sc-100737, FBXO42 (FL-6), is recommended and warrantied for IP applications. Unfortunately we do not have any citations in IP at this time. You can find the recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Tech Service
Date published: 2019-08-07

For Western Blot, is it recommended to use denatured or non-denatured conditions with FBXO42 (FL-6): sc-100737 antibody?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Works well for WB in PANC1 line. Distinct band observed, though also mixed with a ghost non-specific band at ~75 KDa. Tested FBXO42 shRNA knockdown, CRISPR-knockout (homozygous frameshift deletion), and FBXO42-overexpressed (via Lentivirus) clonal stable PANC1 lines. Used this Ab (Lot #: B1220) for WB (1:800 in 5% milk-TBST0.1%, 0.125 ug/mL final, overnight ~10 hours) to test PANC1 cell lysates. 20 ug total proteins of cell lysates were each resolved on a Tris-Glycin gel (4-12% gradient) and transferred to a PVDF membrane. Antibody was freshly diluted in milk without freeze-n-thawing.
Date published: 2020-04-21
Rated 5 out of 5 by from wonderful antibody 1:200 diluted for wb of rat protein,the band is quite strong and special!
Date published: 2017-12-30
Rated 4 out of 5 by from Positive signal in WB in human transfected Positive signal in WB in human transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-04-13
Rated 4 out of 5 by from Good specificity in WB in HeLa whole cell lysate Good specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2013-02-05
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