FATE1 抗体 (B5-9): sc-101220

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- FATE1 抗体 B5-9 是小鼠单克隆 IgG₁ κ，FATE1抗体, 在1篇文献中引用，规格为50 µg/0.5 ml
- 免疫human物种的重组FATE1
- 推荐用于 human 来源的FATE1 WB, IP, IF, IHC(P) 和 ELISA检测
- 关于如何获取FATE1 (B5-9): sc-101220的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成FATE1 抗体 (B5-9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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FATE1 Antibody (B5-9) - Western Blotting - Image 346970

FATE1 Antibody (B5-9) - Immunohistochemistry - Image 36345

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FATE1 Antibody (B5-9) is an IgG₁ κ mouse monoclonal FATE1 antibody (also designated FATE1 antibody) suitable for the detection of the FATE1 protein of human origin by WB, IP, IF, IHC(P) and ELISA. FATE1 Antibody (B5-9) is available as the non-conjugated anti-FATE1 antibody. FATE1 (fetal and adult testis expressed 1), also known as FATE, CT43 (cancer/testis antigen 43) or BJ-HCC-2, is a cancer/testis antigen found in testis and tumor tissues (specifically hepatocarcinoma cells). Its expression is regulated by SF-1 (Steroidogenic Factor 1) and WT1 (Wilms′ tumor protein), two proteins involved in tumorigenesis, suggesting a role for FATE1 in tumor development. FATE1 is exclusively expressed in testis of the 6-11 week old fetus (around the period of gonadal sex differentiation). At 7 weeks, FATE1 is coexpressed with SRY and may play a role in early testicular differentiation. In adults, although predominantly expressed in testis, FATE1 can also be found in heart, kidney, brain, lung and adrenal gland. Mutations in the gene encoding FATE1 may be contributing factors in male infertility.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

Human FATE is a novel X-linked gene expressed in fetal and adult testis. | Olesen, C., et al. 2001. Mol Cell Endocrinol. 184: 25-32. PMID: 11694338
Mutational analysis of the human FATE gene in 144 infertile men. | Olesen, C., et al. 2003. Hum Genet. 113: 195-201. PMID: 12811541
Identification of two novel CT antigens and their capacity to elicit antibody response in hepatocellular carcinoma patients. | Dong, XY., et al. 2003. Br J Cancer. 89: 291-7. PMID: 12865919
Purification and refolding of a novel cancer/testis antigen BJ-HCC-2 expressed in the inclusion bodies of Escherichia coli. | Yang, XA., et al. 2004. Protein Expr Purif. 33: 332-8. PMID: 14711522
Immunohistochemical analysis of the expression of FATE/BJ-HCC-2 antigen in normal and malignant tissues. | Yang, XA., et al. 2005. Lab Invest. 85: 205-13. PMID: 15580283
Increased steroidogenic factor-1 dosage triggers adrenocortical cell proliferation and cancer. | Doghman, M., et al. 2007. Mol Endocrinol. 21: 2968-87. PMID: 17761949

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	FATE1	Entrez Gene ID: 89885	Xq28	NM_033085	UniProt ID: Q969F0	300450

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗FATE1抗体(B5-9)在各种科学出版物中有1篇引文。

针对哺乳动物靶标蛋白的一抗，如抗FATE1 抗体 (B5-9)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

FATE1 抗体 (B5-9) Specifications

替代产品名称

FATE1

产品克隆名称

B5-9

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

2-101

抗原表位靶标

FATE1

抗原表位种属

human

产品反应性/检测

FATE1

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

1篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗FATE1抗体(B5-9)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

Can sc-101220: FATE1 (B5-9) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt

Thank you for your question. Yes, sc-101220: FATE1 (B5-9) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 4 out of 5 by u1060 from Marche bien en WB C'était un échantillon à tester. On en est très content.

Date published: 2017-10-16

Rated 3 out of 5 by CJ from Decent immunoperoxidase membrane and cytoplasmic Decent immunoperoxidase membrane and cytoplasmic staining in formalin-fixed, paraffin-embedded human testis tissue. -SCBT QC

Date published: 2013-08-31

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FATE1 抗体 (B5-9): sc-101220

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References:

FATE1 抗体 (B5-9) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

Can sc-101220: FATE1 (B5-9) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?