关于如何获取Factor VII (CaFVII-22): sc-101369的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP (小鼠IgG Fc 结合蛋白偶联辣根过氧化物酶(HRP)) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) 是 Factor VII Antibody (CaFVII-22) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供Factor VII Antibody (CaFVII-22) (见下方订购信息).
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Factor VII Antibody (CaFVII-22) is a high quality monoclonal Factor VII antibody (also designated Factor VII antibody) suitable for the detection of the Factor VII protein of human origin. Factor VII Antibody (CaFVII-22) is available as both the non-conjugated anti-Factor VII antibody form, as well as multiple conjugated forms of anti-Factor VII antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII) that are involved in a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Factor VII (serum prothrombin conversion accelerator, proconvertin, F7, coagulation factor 7) is a 406 amino acid, vitamin K-dependent, single chain serine protease that is synthesized in the liver and circulates as an inactive precursor. Factor IX A, Factor X A, Factor XII A or thrombin mediated proteolytic cleavage of Factor VII at Arg 152-Ile 153 generates Factor VII A, an active serine protease composed of a catalytic heavy chain disulfide linked to a light chain, containing two EGF-like domains. Mutations at the F7 locus that lead to Factor VII deficiencies are generally asymptomatic or phenotypically uncharacterized, with hemorrhagic diathesis occurring at extremely low levels.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
I have a construction that has a FVII gla domain at C terminal position and I would like to see if it has been gama carboxylated. Can this antibody be useful to detect the gama carboxylation of this domain?
Asked by: Luciano
Thank you for your question. The exact epitope of Factor VII Antibody (CaFVII-22): sc-101369 on its target protein has not been mapped yet, so there is no information that would indicate whether or not the antibody would recognize the C-terminus specifically or differentiate between carboxylated and non-carboxylated FVII.
Please contact our Technical Service Department to arrange a free 10 µg sample of Factor VII (CaFVII-22): sc-101369 antibody to try it out in your experimental setup.
Answered by: Tech Support Europe
Date published: 2020-06-25
Do you have any data, whether the antibody might also recognize murine F7?
Asked by: mabeln
Thank you for your question. Factor VII Antibody (CaFVII-22): sc-101369 is only recommended for detection of the Gla-domain of Factor VII of human origin by WB, IF, IHC(P) and ELISA. This recommendation is based on our tests with this antibody in our labs using our standard protocols.
However, there is a publication from 2018 in which this antibody has been used successfully to detect F7 in murine Schwannoma cells: PMID 29292076.
Answered by: Tech Support Europe
Date published: 2019-07-24
I am using Factor VII (CaFVII-22): sc-101369 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?
Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: