Factor VII抗体 (CaFVII-22) 推荐用于 human 来源的the Gla-domain of Factor VII WB, IF, IHC(P) 和 ELISA检测

Factor VII 抗体 (CaFVII-22): sc-101369

Factor VII抗体 (CaFVII-22) is rated 4.5 out of 5 by 2.
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Datasheets

    • 抗-Factor VII 抗体 CaFVII-22 是小鼠单克隆 IgG1 κ,Factor VII抗体, 在1篇文献中引用,规格为200 µg/ml
    • 免疫human物种的Factor VII
    • Factor VII 抗体 (CaFVII-22) 推荐用于WB, IF, IHC(P) 和 ELISA,检测human 来源的 the Gla-domain of Factor VII
    • 抗-Factor VII 抗体 (CaFVII-22) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Factor VII (CaFVII-22): sc-101369免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

I have a construction that has a FVII gla domain at C terminal position and I would like to see if it has been gama carboxylated. Can this antibody be useful to detect the gama carboxylation of this domain? Thanks

Asked by: Luciano
Thank you for your question. The exact epitope of Factor VII Antibody (CaFVII-22): sc-101369 on its target protein has not been mapped yet, so there is no information that would indicate whether or not the antibody would recognize the C-terminus specifically or differentiate between carboxylated and non-carboxylated FVII. Please contact our Technical Service Department to arrange a free 10 µg sample of Factor VII (CaFVII-22): sc-101369 antibody to try it out in your experimental setup.
Answered by: Tech Support Europe
Date published: 2020-06-25

Do you have any data, whether the antibody might also recognize murine F7?

Asked by: mabeln
Thank you for your question. Factor VII Antibody (CaFVII-22): sc-101369 is only recommended for detection of the Gla-domain of Factor VII of human origin by WB, IF, IHC(P) and ELISA. This recommendation is based on our tests with this antibody in our labs using our standard protocols. However, there is a publication from 2018 in which this antibody has been used successfully to detect F7 in murine Schwannoma cells: PMID 29292076.
Answered by: Tech Support Europe
Date published: 2019-07-24

I am using Factor VII (CaFVII-22): sc-101369 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice for IP images We used this antibody for Immunoperoxidase staining. We were able to take nice images of the stained cells
Date published: 2017-02-02
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