EphB3抗体 (7E5) 推荐用于 mouse, rat 和 human 来源的EphB3 WB, IP, IF, IHC(P) 和 ELISA检测

EphB3 抗体 (7E5): sc-100299

EphB3抗体 (7E5) is rated 4.6 out of 5 by 5.
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Datasheets

    • EphB3抗体(7E5)是小鼠单克隆IgG1 (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组EphB3
    • 推荐用于 mouse, rat 和 human 来源的EphB3 WB, IP, IF, IHC(P) 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
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Can sc-100299: EphB3 (7E5) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, sc-100299: EphB3 (7E5) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Produced good immunoperoxidase membrane staining Produced good immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-04-16
Rated 4 out of 5 by from Positive detection of endogenous EphB3 protein Positive detection of endogenous EphB3 protein by WB using HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from Publishable data obtained by WB and IHC using Publishable data obtained by WB and IHC using mouse bone marrow. -SCBT Publication Review
Date published: 2015-02-16
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human EphB3 protein via Western Blot analysis. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from An increase in signal was observed via Western An increase in signal was observed via Western Blot when comparing mouse EphB3 transfected 293T cells with those that have not been transfected to over express the protein of interest. -SCBT QC
Date published: 2015-01-19
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