ENC1 抗体 (SS-9): sc-100318

ENC1 Antibody (SS-9) is an IgG₁ mouse monoclonal ENC1 antibody (also designated ENC1 antibody) that detects the ENC1 protein of human origin by WB, IP and ELISA. ENC1 Antibody (SS-9) is available as the non-conjugated anti-ENC1 antibody. Proteolytic degradation by the ubiquitin (Ub) system is essential for normal cell cycle progression, cellular differentiation and stress responses. The E3 ubiquitin-protein ligase complex uses a substrate-specific adapter, ENC1 (Ectoderm-neural cortex protein 1), to mediate ubiquitination. ENC1, also known as NRPB or PIG10, is a 589 amino acid Actin-binding protein that is involved in differentiation of neural crest cells and regulation of neuronal process formation. ENC1 is localized to the nuclear matrix and is highly expressed in adult brain and spinal cord tissues. Expression of ENC1 is up-regulated during neuronal differentiation. ENC1 may be regulated by the β-catenin/TCF pathway and is thought to play a role in histogenesis. ENC1 interacts with hypophosphorylated Rb (Retinoblastoma-associated protein) to form a complex that contains CUL-3, Rbx1 and ENC1 which is essential for neuronal cell differentiation. ENC1 contains one BTB (POZ) domain and six Kelch repeats. The BTB domain is thought to be necessary for the protein-protein interactions involved in cytoskeletal organization and the Kelch repeats denote a conserved tertiary structure. ENC1 is highly expressed in brain tumors, suggesting a possible role in carcinogenesis.

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References:

1. Assignment of the ectodermal-neural cortex 1 gene (ENC1) to human chromosome band 5q13 by in situ hybridization.  |  Hernandez, MC., et al. 1999. Cytogenet Cell Genet. 87: 89-90. PMID: 10640818
2. Assignment of the ectodermal-neural cortex 1 gene (Enc1) to mouse chromosome band 13D1 by fluorescence in situ hybridization.  |  Hernandez, M., et al. 2000. Cytogenet Cell Genet. 89: 158-9. PMID: 10965111
3. Up-regulation of the ectodermal-neural cortex 1 (ENC1) gene, a downstream target of the beta-catenin/T-cell factor complex, in colorectal carcinomas.  |  Fujita, M., et al. 2001. Cancer Res. 61: 7722-6. PMID: 11691783
4. Disruption of a novel ectodermal neural cortex 1 antisense gene, ENC-1AS and identification of ENC-1 overexpression in hairy cell leukemia.  |  Hammarsund, M., et al. 2004. Hum Mol Genet. 13: 2925-36. PMID: 15459180
5. Pallial expression of Enc1 RNA in postnatal mouse telencephalon.  |  García-Calero, E. and Puelles, L. 2005. Brain Res Bull. 66: 445-8. PMID: 16144629
6. A model for p53-induced apoptosis.  |  Polyak, K., et al. 1997. Nature. 389: 300-5. PMID: 9305847
7. NRP/B, a novel nuclear matrix protein, associates with p110(RB) and is involved in neuronal differentiation.  |  Kim, TA., et al. 1998. J Cell Biol. 141: 553-66. PMID: 9566959
8. Cloning of human ENC-1 and evaluation of its expression and regulation in nervous system tumors.  |  Hernandez, MC., et al. 1998. Exp Cell Res. 242: 470-7. PMID: 9683534