eIF4GII 抗体 (R16S): sc-100732

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- eIF4GII 抗体 R16S 是小鼠单克隆 IgG_2b κ，eIF4GII抗体, 在2篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组eIF4GII
- 推荐用于 human 来源的eIF4GII WB, IP, IF, IHC(P) 和 ELISA检测
- 关于如何获取eIF4GII (R16S): sc-100732的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成eIF4GII 抗体 (R16S)的首选二抗检测试剂的鉴定。这项工作正在进行中。

SEE ALSO...

此外 eIF 抗体包括 eIF1, eIF1AY, eIF2 α, eIF2B α, eIF2B β, eIF2B δ, eIF2B ε, eIF2 β, eIF2B γ和 eIF2C
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eIF4GII Antibody (R16S) - Western Blotting - Image 39765

eIF4GII Antibody (R16S) - Immunohistochemistry - Image 36136

eIF4GII Antibody (R16S) - Western Blotting - Image 34105

eIF4GII Antibody (R16S) - Western Blotting - Image 54191

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eIF4GII Antibody (R16S) is an IgG_2b κ mouse monoclonal eIF4GII antibody (also designated EIF4G3 antibody) that detects the eIF4GII protein of human origin by WB, IP, IF, IHC(P) and ELISA. eIF4GII Antibody (R16S) is available as the non-conjugated anti-eIF4GII antibody. Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a 5′-cap motif and recruits the 43S ribosomal preinitiation complex to the transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5′ cap, eIF4A is a bidirectional RNA helicase, and eIF4GI and eIF4GII are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3 and the 40S ribosome. eIF4GII (also known as eIF4G3 and eIF4-g3) is a 1,585 amino acid protein that is 46% homologous and functionally similar to eIF4GI.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation. | Gingras, AC., et al. 1999. Annu Rev Biochem. 68: 913-63. PMID: 10872469
A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate in vivo. | Asano, K., et al. 2000. Genes Dev. 14: 2534-46. PMID: 11018020
Bidirectional RNA helicase activity of eucaryotic translation initiation factors 4A and 4F. | Rozen, F., et al. 1990. Mol Cell Biol. 10: 1134-44. PMID: 2304461
Initiation of protein synthesis in eukaryotic cells. | Pain, VM. 1996. Eur J Biochem. 236: 747-71. PMID: 8665893
A novel functional human eukaryotic translation initiation factor 4G. | Gradi, A., et al. 1998. Mol Cell Biol. 18: 334-42. PMID: 9418880
A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation. | Imataka, H., et al. 1998. EMBO J. 17: 7480-9. PMID: 9857202

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	EIF4G3	Entrez Gene ID: 8672	1p36.12	XM_011542356, XM_011542361, XM_011542370, XM_011542371, XM_011542374, XM_011542375, XM_011542381, XM_017002672, XM_017002673, XM_017002674, XM_017002675, XM_017002676, XM_017002677, XM_017002678, XM_017002679, XM_017002680, XM_017002681, XM_017002682, XM_017002683, XM_017002684, XM_017002685, XM_017002686, XM_017002687, XM_017002688, XM_017002689, XM_017002690, XM_017002691, XM_017002692, XM_017002693, XM_017002694, XM_017002695, XM_017002696, XM_017002697, XM_017002698, XM_017002699, XM_017002700, XM_017002701, XM_017002702, XM_017002703, XM_017002704, XM_017002705, XM_017002706, XM_017002707, XM_017002708, XM_017002709, XM_017002710, XM_017002711, XM_017002712, NM_001198801, NM_001198802, NM_001198803, NM_003760	UniProt ID: O43432	603929

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗eIF4GII抗体(R16S)在各种科学出版物中有2篇引文。

针对哺乳动物靶标蛋白的一抗，如抗eIF4GII 抗体 (R16S)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

eIF4GII 抗体 (R16S) Specifications

替代产品名称

EIF4G3

产品克隆名称

R16S

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG_2b kappa light chain

氨基酸

1-516

抗原表位靶标

eIF4GII

抗原表位种属

human

产品反应性/检测

eIF4GII

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

2篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗eIF4GII抗体(R16S)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

Can sc-100732: eIF4GII (R16S) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin

Thank you for your question. Yes, sc-100732: eIF4GII (R16S) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 5 out of 5 by QZ from Wonderful for paraffin Wonderful for paraffin-embedded human lung samples. -SCBT QC

Date published: 2015-05-01

Rated 4 out of 5 by LR from This antibody performs well in Western Blot This antibody performs well in Western Blot, positive in MCF7 cell lysates -SCBT QC

Date published: 2014-12-18

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