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Eps15 homology domain (EHD)-containing proteins function in the exit of receptors and other membrane proteins from the endosomal recycling compartment. EHD2 (EH-domain containing 2), also known as PAST2, is a 543 amino acid protein that contains one EF-hand domain and one EH domain. Expressed at high levels in heart and at lower levels in lung, placenta and skeletal muscle, EHD2 interacts with various proteins such as the glucose transporter Glut4 and the endocytotic-associated protein EHBP1. When EHD2 associates with insulin-induced Glut4, it can recruit Glut4 to the plasma membrane, thereby allowing Glut4 to bind glucose and regulate blood sugar levels. Additionally, EHD2 interacts with EHBP1 and is thought to link EHBP1-associated endocytotic events with actin cytoskeleton dynamics. Through its interactions with these two proteins, EHD2 is involved in both maintaining blood glucose levels and mediating actin-associated endocytosis.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using EHD2 (L-05): sc-100724 for immunofluorescence, what dilution of this antibody should I use?
Asked by: jenni
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here:
Answered by: Technical Support
Date published: 2017-03-24
I have an strong band around 50kDa in two of my conditions meanwhile i have at 70 in the control. There is an hypothetic isoform of EHD2 of a delection of the starting part of EHD2 which region this antibody is recognising? There is know unespecificties?
Asked by: Curenama
Thank you for your question. This mouse monoclonal, EHD2 Antibody (L-05): sc-100724, is raised against recombinant EHD2 of human origin. The exact epitope recognized by this monoclonal antibody has not been determined, however the immunogen is mapping between amino acids 350-450 of human EHD2. If you have additional questions about the reactivity of this antibody, please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
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