EHD2抗体 (L-05) 推荐用于 mouse, rat 和 human 来源的EHD2 WB, IP, IF 和 ELISA检测

EHD2 抗体 (L-05): sc-100724

EHD2抗体 (L-05) is rated 4.0 out of 5 by 2.
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Datasheets

    • EHD2抗体(L-05)是小鼠单克隆IgG1 κ, 在4篇文献中引用,规格为100 µg/ml
    • 免疫human物种的重组EHD2
    • 推荐用于 mouse, rat 和 human 来源的EHD2 WB, IP, IF 和 ELISA检测
    • 目前,我们还没有完成EHD2 抗体 (L-05)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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Hello , have you try this antibody in zebrafish tissues? I would want to try it. Thank you so much

Asked by: MariaGCNIC
Thank you for your question. Anti-EHD2 Antibody (L-05): sc-100724 is only recommended for detection of EHD2 of mouse, rat and human origin by WB, IP, IF and ELISA. We do not have any data on its reactivity with Zebrafish samples.
Answered by: Tech Support Europe
Date published: 2021-09-23

I am using EHD2 (L-05): sc-100724 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenniferc
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24

I have an strong band around 50kDa in two of my conditions meanwhile i have at 70 in the control. There is an hypothetic isoform of EHD2 of a delection of the starting part of EHD2 which region this antibody is recognising? There is know unespecificties?

Asked by: Curenama
Thank you for your question. This mouse monoclonal, EHD2 Antibody (L-05): sc-100724, is raised against recombinant EHD2 of human origin. The exact epitope recognized by this monoclonal antibody has not been determined, however the immunogen is mapping between amino acids 350-450 of human EHD2. If you have additional questions about the reactivity of this antibody, please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2016-12-14
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Rated 4 out of 5 by from Robust immunofluorescence nuclear staining Robust immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-05-15
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