EHD2抗体 (L-05) 推荐用于 mouse, rat 和 human 来源的EHD2 WB, IP, IF 和 ELISA检测

EHD2 抗体 (L-05): sc-100724

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EHD2 抗体 (L-05) is rated 4.0 out of 5 by 2.
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Datasheets

    • EHD2抗体(L-05)是小鼠单克隆IgG1 (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组EHD2
    • 推荐用于 mouse, rat 和 human 来源的EHD2 WB, IP, IF 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)

I am using EHD2 (L-05): sc-100724 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenni
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24

I have an strong band around 50kDa in two of my conditions meanwhile i have at 70 in the control. There is an hypothetic isoform of EHD2 of a delection of the starting part of EHD2 which region this antibody is recognising? There is know unespecificties?

Asked by: Curenama
Thank you for your question. This mouse monoclonal, EHD2 Antibody (L-05): sc-100724, is raised against recombinant EHD2 of human origin. The exact epitope recognized by this monoclonal antibody has not been determined, however the immunogen is mapping between amino acids 350-450 of human EHD2. If you have additional questions about the reactivity of this antibody, please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2016-12-14
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