EGR1 抗体 (S-25): sc-101033

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Datasheets

- EGR1抗体(S-25)是小鼠单克隆IgG_2a κ, 在20篇文献中引用，规格为100 µg/ml
- raised against a partial recombinant protein mapping within amino acids 444-544 of Egr-1 of human origin
- 推荐用于 mouse, rat 和 human 来源的Egr-1 p82 WB, IP, IF, IHC(P) 和 ELISA检测
- 关于如何获取EGR1 (S-25): sc-101033的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成EGR1 抗体 (S-25)的首选二抗检测试剂的鉴定。这项工作正在进行中。

SEE ALSO...

此外 Egr 抗体包括 Egr-1, Egr-2和 Egr-3
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

Egr-1 Antibody (S-25) - Western Blotting - Image 34398

Egr-1 Antibody (S-25) - Immunohistochemistry - Image 36265

Egr-1 Antibody (S-25) - Immunofluorescence - Image 35768

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Egr-1 Antibody (S-25) is an IgG2a κ mouse monoclonal Egr-1 antibody that detects Egr-1 p82 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Egr-1 Antibody (S-25) is available as the non-conjugated anti-Egr-1 antibody form. Egr-1, Egr-2, Egr-3 and Egr-4 are nuclear transcription factors belonging to the Egr C2H2-type zinc-finger protein family and containing three C2H2-type zinc fingers. As immediate early proteins, Egr transcription factors are rapidly induced by diverse extracellular stimuli. They are subject to tight differential control through diverse mechanisms at several levels of regulation: transcriptional; translational and posttranslational (including glycosylation, phosphorylation and redox) mechanisms; and protein-protein interaction. Egr-1 binds to the DNA sequence 5′-CGCCCCCGC-3′ (Egr-site), thereby activating transcription of target genes whose products are required for mitogenesis and differentiation. Egr-2 binds specific DNA sites located in the promoter region of HoxA4. Egr-2 defects cause congenital hypo-myelination neuropathy (also designated Charcot-Marie-Tooth disease) and Dejerine-Sottas neuropathology (also designated hereditary motor and sensory neuropathy III). Egr-3 is involved in muscle spindle development and is expressed in T cells 20 minutes following activation. Egr-4 binds to the Egr consensus motif GCGTGGGCG, functions as a transcriptional repressor, and displays autoregulatory activities, downregulating its own gene promoter in a dose dependent manner.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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References:

Egr-1 is activated by 17beta-estradiol in MCF-7 cells by mitogen-activated protein kinase-dependent phosphorylation of ELK-1. | Chen, CC., et al. 2004. J Cell Biochem. 93: 1063-74. PMID: 15449318
Stretch-inducible expression of the angiogenic factor CCN1 in vascular smooth muscle cells is mediated by Egr-1. | Grote, K., et al. 2004. J Biol Chem. 279: 55675-81. PMID: 15492009
HGF induces fibronectin matrix synthesis in melanoma cells through MAP kinase-dependent signaling pathway and induction of Egr-1. | Gaggioli, C., et al. 2005. Oncogene. 24: 1423-33. PMID: 15608673
Essential interaction of Egr-1 at an islet-specific response element for basal and gastrin-dependent glucagon gene transactivation in pancreatic alpha-cells. | Leung-Theung-Long, S., et al. 2005. J Biol Chem. 280: 7976-84. PMID: 15611055
The MAPK pathway and Egr-1 mediate stress-related behavioral effects of glucocorticoids. | Revest, JM., et al. 2005. Nat Neurosci. 8: 664-72. PMID: 15834420
Egr transcription factors in the nervous system. | Beckmann, AM. and Wilce, PA. 1997. Neurochem Int. 31: 477-510; discussion 517-6. PMID: 9307998
The human zinc finger protein EGR-4 acts as autoregulatory transcriptional repressor. | Zipfel, PF., et al. 1997. Biochim Biophys Acta. 1354: 134-44. PMID: 9396630

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	EGR1	Entrez Gene ID: 1958	5q31.2	NM_001964	UniProt ID: P18146	128990
Mouse	Egr1	Entrez Gene ID: 13653	18 B1	NM_007913	UniProt ID: P08046	N/A

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗EGR1抗体(S-25)在各种科学出版物中有20篇引文。

针对哺乳动物靶标蛋白的一抗，如抗EGR1 抗体 (S-25)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

EGR1 抗体 (S-25) Specifications

替代产品名称

Egr-1

产品克隆名称

S-25

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG_2a kappa light chain

氨基酸

444-544

抗原表位靶标

Egr-1

抗原表位种属

human

产品反应性/检测

Egr-1 p82

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

20篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗EGR1抗体(S-25)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero

We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety

Answered by: Technical Support 8

Date published: 2017-01-09

Rated 3 out of 5 by JDAlbarnaz from It works for western blotting. It detected a band with the expected molecular mass of EGR1 in total protein extracts from HeLa cells (1:500 in 5% milk/TBS-0.1% Tween, overnight at 4C).

Date published: 2018-02-15

Rated 1 out of 5 by Just15 from Didnot work for WB No band detected in MDA-MB-231 cell line. Used the antibody at 1:1000 dilution

Date published: 2017-06-07

Rated 5 out of 5 by DefinitelyNotMatt from Reliable Antibody, Great for WB This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Great for WB.

Date published: 2017-01-13

Rated 5 out of 5 by CJ from The results of western blot were great The results of western blot were great.

Date published: 2015-12-04

Rated 4 out of 5 by Yu from This has worked well in WB&IF This has worked well in WB&IF.

Date published: 2015-10-21

Rated 5 out of 5 by MC from Favorable signal achieved in Raw 264 Favorable signal achieved in Raw 264.7 whole cell lysate. -SCBT QC

Date published: 2015-05-14

Rated 3 out of 5 by MS from Produced acceptable immunoperoxidase nuclear Produced acceptable immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human placenta tissue. -SCBT QC

Date published: 2015-05-08

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