EGFR抗体 (R-1) 推荐用于 mouse, rat 和 human 来源的EGFR WB, IP, IF 和 FCM检测

EGFR 抗体 (R-1): sc-101

EGFR抗体 (R-1) is rated 4.9 out of 5 by 9.
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Datasheets

    • EGFR抗体(R-1)是小鼠单克隆IgG2b (kappa轻链),规格为200 µg/ml
    • raised against human epidermoid carcinoma cell line A431, with epitope mapping between amino acids 6-273 of human EGFR
    • 抗-EGFR 抗体 (R-1) 推荐用于 WB, IP, IF 和 FCM,检测mouse, rat 和human 来源的 EGFR
    • 抗-EGFR 抗体 (R-1) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 还可偶联PerCP , PerCP-Cy5.5 以及 Alexa Fluor® 405 用于IF, IHC(P) and FCM
    • also provided azide-free for biological studies, sc-101 L, 200 µg/0.1 ml
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取EGFR (R-1): sc-101免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Can I get a free sample of an antibody (sc-365829) to try for my application?  Can I try several of your EGF-R antibodies to determine the optimal reagent for my use (sc-120 & sc-101)? Thank You

Asked by: MAntczak
We currently offer sample sizes for all 3 of these EGFR antibodies. Please contact our Technical Service Department for current information on how to receive a free 10 ug monoclonal antibody sample.
Answered by: Technical Support
Date published: 2020-04-09

bonjour,  savez-vous si la reconnaissance de la protéine par l'anticorps est dépendante des N-glycosylations de l'EGFR ?

Asked by: Serge
merci pour votre question. Veuillez contacter notre Service Technique par email [email protected] pour plus d'informations sur ce produit.
Answered by: Tech Support Europe
Date published: 2019-12-16

How can EGFR Antibody (R-1): sc-101 be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. EGFR Antibody (R-1): sc-101 is currently available conjugated to phycoerythrin (sc-101 PE), fluoroscein (sc-101 FITC), Alexa Fluor® 488 (sc-101 AF488), Alexa Fluor® 647 (sc-101 AF647), PerCP (sc-101 PerCP) or Alexa Fluor® 405 (sc-101 AF405)
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 4 out of 5 by from Worked well Gave nice strong bands for WB at 1:200. Used with mouse samples.
Date published: 2018-03-22
Rated 5 out of 5 by from A good choice for EGFR detection This antibody is a great choice. The IF data is features strong and specific signals while Western blots will result in bright clean bands. Researchers have published with this antibody over 30 times.
Date published: 2017-01-17
Rated 5 out of 5 by from Outstanding!! Great IF and western blot results;far superior to polyclonal alternatives
Date published: 2017-01-06
Rated 5 out of 5 by from no comment no comment
Date published: 2015-05-15
Rated 5 out of 5 by from Worked well for Western blot with HUVEC samples Worked well for Western blot with HUVEC samples. -SCBT Publication Review
Date published: 2015-03-27
Rated 5 out of 5 by from IHC publication with human esophagus adenocarcinoma. -SCBT Publication Review
Date published: 2015-02-13
Rated 5 out of 5 by from Great antibody for mouse IP Great antibody for mouse IP, published using MCF-7. -SCBT Publication Review
Date published: 2015-01-29
Rated 5 out of 5 by from Worked great for Flow Cytometry on A Worked great for Flow Cytometry on A-431 human samples. -SCBT QC
Date published: 2015-01-27
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