EF-1 gamma抗体 (X5-P) 推荐用于 mouse, rat 和 human 来源的EF-1 γ WB, IP, IF 和 ELISA检测

EF-1 γ 抗体 (X5-P): sc-101035

EF-1 γ抗体 (X5-P) is rated 5.0 out of 5 by 6.
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Datasheets

    • EF-1 γ抗体(X5-P)是小鼠单克隆IgG1 (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组EF-1 γ
    • 推荐用于 mouse, rat 和 human 来源的EF-1 γ WB, IP, IF 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)

I am using EF-1 γ (X5-P): sc-101035 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good specificity and signal strength in WB in non Good specificity and signal strength in WB in non-transfected 293T, human EF-1 transfected 293T and JAR whole cell lysates. -SCBT QC
Date published: 2015-06-13
Rated 5 out of 5 by from Produced exceptional immunofluorescence cytoplasmic Produced exceptional immunofluorescence cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using mouse EF-1 transfected 293T and JAR whole cell lysates. -SCBT QC
Date published: 2015-05-05
Rated 5 out of 5 by from Good antibody for western Good antibody for western, clean background and specific band in HL-60 whole cell lysate. - SCBT QC
Date published: 2015-04-30
Rated 5 out of 5 by from Great Great, strong band observed in WB in K-562 whole cell lysate. -SCBT QC
Date published: 2015-04-24
Rated 5 out of 5 by from Band detected at expected mw in 293 whole Band detected at expected mw in 293 whole cell lysate. -SCBT QC
Date published: 2015-04-02
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