EBV LMP-2A 抗体 (15F9): sc-101315

4.5(2)

- EBV LMP-2A 抗体 15F9 是大鼠单克隆 IgG₁，EBV LMP-2A抗体, 在6篇文献中引用，规格为200 µg/ml
- raised against bacterial TrpE-LMP2A fusion protein
- EBV LMP-2A 抗体 (15F9) 推荐用于WB, IF 和 IHC(P)，检测EBV 来源的 LMP-2A
- 抗-EBV LMP-2A 抗体 (15F9) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
- 还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
- 还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
- 关于如何获取EBV LMP-2A (15F9): sc-101315的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成EBV LMP-2A 抗体 (15F9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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此外 EBV 抗体包括 EBV Bam HI Z, EBV Bcl-2, EBV Ea-D, EBV EBNA-1, EBV gp110 Envelope Protein, EBV gp350 Envelope Protein, EBV LMP-1, EBV LMP-2A, EBV viral capsid antigen和 EBV ZEBRA
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

EBV LMP-2A Antibody (15F9) is an IgG₁ rat monoclonal EBV LMP-2A antibody (also designated Human herpesvirus 4 LMP2A antibody, Latent Membrane Protein 2A (LMP2A) antibody, LMP2A transmembrane protein antibody, HHV-4 LMP2A antibody, or Epstein-Barr virus antibody) suitable for the detection of the EBV LMP-2A protein of EBV origin by WB, IF and IHC(P). EBV LMP-2A Antibody (15F9) is available as both the non-conjugated anti-EBV LMP-2A antibody form, as well as multiple conjugated forms of anti-EBV LMP-2A antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Epstein-Barr virus (EBV) is a human herpesvirus that ubiquitously infects the majority of the human population. Although most EBV infections are asymptomatic, the virus itself is associated with epithelioid and lymphoid malignancies. EBV LMP-2A (Epstein-Barr virus latent membrane protein 2A), also known as LMP2A, is a 497 amino acid EBV protein that exists on the surface of EBV-infected cells and consists of a short C-terminal tail, 12 membrane-spanning domains and a long N-terminal segment. Expressed in most EBV-associated malignancies, EBV LMP-2A associates with protein tyrosine kinases and, via this association, activates the PI 3-kinase (phosphatidylinositol 3-kinase) pathway, thereby preventing apoptosis. Through its ability to regulate PI 3-kinase signaling events, EBV LMP-2A provides growth and developmental survival signals to infected cells, thus facilitating EBV-induced tumorigenesis. Additionally, EBV LMP-2A can mimic B-cell receptor complex (BCR) signaling in BCR-negative cells, further implicating EBV LMP-2A as an important factor in the survival of mutated cells.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

EBV latent membrane protein 2A induces autoreactive B cell activation and TLR hypersensitivity. | Wang, H., et al. 2006. J Immunol. 177: 2793-802. PMID: 16920914
Epstein-barr virus latent membrane protein 2B (LMP2B) modulates LMP2A activity. | Rovedo, M. and Longnecker, R. 2007. J Virol. 81: 84-94. PMID: 17035319
Cholesterol is critical for Epstein-Barr virus latent membrane protein 2A trafficking and protein stability. | Ikeda, M. and Longnecker, R. 2007. Virology. 360: 461-8. PMID: 17150237
Epstein-Barr virus latent membrane protein 2A mediates transformation through constitutive activation of the Ras/PI3-K/Akt Pathway. | Fukuda, M. and Longnecker, R. 2007. J Virol. 81: 9299-306. PMID: 17582000
Epstein-Barr virus latent membrane protein 2A is a B-cell receptor mimic and essential for B-cell survival. | Mancao, C. and Hammerschmidt, W. 2007. Blood. 110: 3715-21. PMID: 17682125
Acetylated histone H3 and H4 mark the upregulated LMP2A promoter of Epstein-Barr virus in lymphoid cells. | Gerle, B., et al. 2007. J Virol. 81: 13242-7. PMID: 17898065
An auto-regulatory loop for EBV LMP2A involves activation of Notch. | Anderson, LJ. and Longnecker, R. 2008. Virology. 371: 257-66. PMID: 17980397
Survival advantage of EBV-associated gastric carcinoma: survivin up-regulation by viral latent membrane protein 2A. | Hino, R., et al. 2008. Cancer Res. 68: 1427-35. PMID: 18316606
Identification of latent membrane protein 2A (LMP2A) domains essential for the LMP2A dominant-negative effect on B-lymphocyte surface immunoglobulin signal transduction. | Fruehling, S., et al. 1996. J Virol. 70: 6216-26. PMID: 8709248

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针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗EBV LMP-2A抗体(15F9)在各种科学出版物中有6篇引文。

针对哺乳动物靶标蛋白的一抗，如抗EBV LMP-2A 抗体 (15F9)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

EBV LMP-2A 抗体 (15F9) Specifications

替代产品名称

Epstein–Barr virus LMP2A

产品克隆名称

15F9

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁

氨基酸

抗原表位靶点

EBV LMP-2A

抗原表位种属

EBV

产品反应性/检测

LMP-2A

种属反应性/检测

EBV

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IF, IHC(P)

瓶的信息

Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

6篇各种科学出版物的引文

可选的偶联物/形式

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase PE: Phycoerythrin

针对非哺乳动物靶点的一抗，如抗EBV LMP-2A抗体 (15F9)，在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼，非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗EBV LMP-2A抗体推荐用于以下应用：WB (Western blotting), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和 TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

Can EBV LMP-2A (15F9): sc-101315 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, EBV LMP-2A (15F9): sc-101315 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.

Answered by: Technical Support

Date published: 2020-06-13

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