DPH2 抗体 (6E7): sc-101200

DPH2 Antibody (6E7) is an IgG_2a κ mouse monoclonal DPH2 antibody (also designated DPH2 antibody) that detects the DPH2 protein of mouse, rat and human origin by WB and IF. DPH2 Antibody (6E7) is available as the non-conjugated anti-DPH2 antibody. DPH2 (diphthamide biosynthesis protein 2), also known as DPH2L2, is a 489 amino acid protein that shows strong expression in skeletal muscle; moderate expression in heart, small intestine, liver, pancreas, testis and colon; and lesser expression in brain, placenta, kidney, spleen, thymus, prostate, ovary and lymphocytes. DPH2 interacts with DPH1 and, functioning together as a dimer or multimer, DPH1 and DPH2 may participate in diphthamide biosynthesis. Diphthamide is a posttranslationally modified histidine residue which occurs in EF-2 (elongation factor 2) and targets diphtheria toxin ADP-ribosylation. The loss of DPH2 in Saccharomyces cerevisiae is believed to suppress zymocicity. Two transcript variants encoding different isoforms have been found for this gene.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Elongator′s toxin-target (TOT) function is nuclear localization sequence dependent and suppressed by post-translational modification.  |  Fichtner, L., et al. 2003. Mol Microbiol. 49: 1297-307. PMID: 12940988
2. Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2.  |  Liu, S., et al. 2004. Mol Cell Biol. 24: 9487-97. PMID: 15485916
3. OVCA1: tumor suppressor gene.  |  Chen, CM. and Behringer, RR. 2005. Curr Opin Genet Dev. 15: 49-54. PMID: 15661533
4. Diphthamide synthesis in Saccharomyces cerevisiae: structure of the DPH2 gene.  |  Mattheakis, LC., et al. 1993. Gene. 132: 149-54. PMID: 8406038
5. A cDNA from the ovarian cancer critical region of deletion on chromosome 17p13.3.  |  Phillips, NJ., et al. 1996. Cancer Lett. 102: 85-90. PMID: 8603384
6. Cloning and localization of a human diphthamide biosynthesis-like protein-2 gene, DPH2L2.  |  Schultz, DC., et al. 1998. Genomics. 52: 186-91. PMID: 9782084