DP-1/3 抗体 (L7-7): sc-101031

DP-1/3 Antibody (L7-7) is an IgG_2a κ mouse monoclonal DP-1/3 antibody (also designated DP-1/3 antibody) that detects the DP-1/3 protein of human origin by WB, IP, IF and ELISA. DP-1/3 Antibody (L7-7) is available as the non-conjugated anti-DP-1/3 antibody. The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 forms heterodimers with a second protein, designated DP-1, forming an "active" E2F transcriptional regulatory complex. Additional members of the E2F family include E2F-2, E2F-3, E2F-4, E2F-5 and DP-2.

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References:

1. E2F: a link between the Rb tumor suppressor protein and viral oncoproteins.  |  Nevins, JR. 1992. Science. 258: 424-9. PMID: 1411535
2. Identification and characterization of novel isoforms of human DP-1: DP-1{alpha} regulates the transcriptional activity of E2F1 as well as cell cycle progression in a dominant-negative manner.  |  Ishida, H., et al. 2005. J Biol Chem. 280: 24642-8. PMID: 15863509
3. A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F.  |  Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
4. Heterogeneous cross-talk of E2F family members is crucially involved in growth modulatory effects of interferon-gamma and EGF.  |  Reimer, D., et al. 2006. Cancer Biol Ther. 5: 771-6. PMID: 16721044
5. Cloning and characterization of human DP2, a novel dimerization partner of E2F.  |  Zhang, Y. and Chellappan, SP. 1995. Oncogene. 10: 2085-93. PMID: 7784053
6. E2F-4, a new member of the E2F transcription factor family, interacts with p107.  |  Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
7. E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo.  |  Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
8. Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members.  |  Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
9. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation.  |  Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995