DD4 抗体 (WW3): sc-100526

DD4 Antibody (WW3) is an IgG₁ κ mouse monoclonal DD4 antibody (also designated DD4 antibody) that detects the DD4 protein of human origin by WB, IP, IF, IHC(P) and ELISA. DD4 Antibody (WW3) is available as the non-conjugated anti-DD4 antibody. Human liver contains isoforms of dihydrodiol dehydrogenase (DD1, DD2, DD3 and DD4), which belong to the aldo-oxo reductase/aldo-keto reductase (AKR) superfamily, have 20α- or 3α-hydroxysteroid dehydrogenase (HSD) activity. DD1 is also designated AKR1C1, DDH or DDH1 while DD2 also can be designated AKR1C2, dDD, BABP or DDH2. AKR1C3 and 3α-HSD are alternate designations for DD3, while DD4 also can be called AKR1C4, CD or CHDR. DD1 and DD2 are 20α-HSDs, whereas DD3 and DD4 are the 3α-HSDs. The multiple human cytosolic dihydrodiol dehydrogenases are involved in the metabolism of xenobiotics, such as polycyclic aromatic hydrocarbons, pesticides and steroid hormones, and are responsible for the reduction of ketone-containing drugs by using NADH or NADPH as a cofactor. The 20α-HSD catalyzes the reaction of progesterone to the inactive form 20α-hydroxyprogesterone. The 3α-HSD is a cytosolic, monomeric, NADPH-dependent oxidoreductase that reduces 3-keto-5-dihydrosteroids to their tetrahydro products. DD1 and DD2 are ubiquitously expressed, whereas DD4 mRNA is restricted to the liver. DD3 is a unique enzyme that can specifically catalyze the dehydrogenation of trans-benzenedihydrodiol and trans-naphthalenedihydrodiol.

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References:

1. Characterization of a novel variant (S145C/L311V) of 3alpha-hydroxysteroid/dihydrodiol dehydrogenase in human liver.  |  Kume, T., et al. 1999. Pharmacogenetics. 9: 763-71. PMID: 10634139
2. Close kinship of human 20alpha-hydroxysteroid dehydrogenase gene with three aldo-keto reductase genes.  |  Nishizawa, M., et al. 2000. Genes Cells. 5: 111-25. PMID: 10672042
3. A novel dihydrodiol dehydrogenase in bovine liver cytosol: purification and characterization of multiple forms of dihydrodiol dehydrogenase.  |  Mizoguchi, T., et al. 1992. J Biochem. 112: 523-9. PMID: 1491006
4. Human hepatic 3 alpha-hydroxysteroid dehydrogenase: possible identity with human hepatic chlordecone reductase.  |  Binstock, JM., et al. 1992. Biochem Biophys Res Commun. 187: 760-6. PMID: 1530633
5. Enzymatic characterization of a novel bovine liver dihydrodiol dehydrogenase--reaction mechanism and bile acid dehydrogenase activity.  |  Nanjo, H., et al. 1995. Biochim Biophys Acta. 1244: 53-61. PMID: 7766669
6. Localization of multiple human dihydrodiol dehydrogenase (DDH1 and DDH2) and chlordecone reductase (CHDR) genes in chromosome 10 by the polymerase chain reaction and fluorescence in situ hybridization.  |  Khanna, M., et al. 1995. Genomics. 25: 588-90. PMID: 7789999
7. Relationship of human liver dihydrodiol dehydrogenases to hepatic bile-acid-binding protein and an oxidoreductase of human colon cells.  |  Hara, A., et al. 1996. Biochem J. 313 (Pt 2): 373-6. PMID: 8573067
8. Sequence of the cDNA of a human dihydrodiol dehydrogenase isoform (AKR1C2) and tissue distribution of its mRNA.  |  Shiraishi, H., et al. 1998. Biochem J. 334 (Pt 2): 399-405. PMID: 9716498