DAZ 抗体 (Z6Q): sc-100705

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- DAZ 抗体 Z6Q 是小鼠单克隆 IgG₁ κ，DAZ抗体, 在1篇文献中引用，规格为100 µg/ml
- raised against recombinant DAZ11 of human origin
- 推荐用于 human 来源的DAZ1, DAZ2, DAZ3 and DAZ4 WB, IP, IF, IHC(P) 和 ELISA检测
- 目前，我们还没有完成DAZ 抗体 (Z6Q)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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此外 DAZ 抗体包括 DAZL
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DAZ Antibody (Z6Q) - Immunohistochemistry - Image 36126

DAZ Antibody (Z6Q) - Western Blotting - Image 34076

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DAZ Antibody (Z6Q) is an IgG₁ κ mouse monoclonal DAZ antibody (also designated DAZ antibody) that detects the DAZ protein of human origin by WB, IP, IF, IHC(P) and ELISA. DAZ Antibody (Z6Q) is available as the non-conjugated anti-DAZ antibody. Spermatogenesis is the process by which male spermatogonia develop into mature spermatozoa. DAZ (deleted in azoospermia) are RNA-binding proteins that play an essential role in spermatogenesis. DAZ proteins influence the first stages of spermatogenesis and the maintenance of germ cell populations. DAZ proteins (DAZ1, DAZ2, DAZ3, DAZ4 and DAZ5) are encoded by separate genes on chromosome Y, each of which contain an AZFc domain in their codinga region. DAZ proteins are localized to the nucleus of spermatogonia, but relocate to the cytoplasm during meiosis. DAZ proteins contain an RRM (RNA recognition motif) domain that may regulate mRNA translation by binding to the 3′UTR. Deletions in the genes encoding DAZ proteins may cause azoospermia or oligospermia which can lead to male infertility.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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References:

Identification of two novel proteins that interact with germ-cell-specific RNA-binding proteins DAZ and DAZL1. | Tsui, S., et al. 2000. Genomics. 65: 266-73. PMID: 10857750
Four DAZ genes in two clusters found in the AZFc region of the human Y chromosome. | Saxena, R., et al. 2000. Genomics. 67: 256-67. PMID: 10936047
Inhibin B plasma concentrations in infertile patients with DAZ gene deletions treated with FSH. | Foresta, C., et al. 2002. Eur J Endocrinol. 146: 801-6. PMID: 12039700
The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes. | Skaletsky, H., et al. 2003. Nature. 423: 825-37. PMID: 12815422
Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA-binding protein gene. | Reijo, R., et al. 1995. Nat Genet. 10: 383-93. PMID: 7670487

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	DAZ1	Entrez Gene ID: 1617	Yq11.223	XM_011531482, XM_011531483, NM_004081	UniProt ID: Q9NQZ3	400003
Human	DAZ2	Entrez Gene ID: 57055	Yq11.223	NM_001005785, NM_001005786, NM_020363	UniProt ID: Q13117	400026
Human	DAZ3	Entrez Gene ID: 57054	Yq11.23	NM_020364	UniProt ID: Q9NR90	400027
Human	DAZ4	Entrez Gene ID: 57135	Yq11.23	XM_005262602, XM_011531509, XM_011531510, NM_001005375, NM_020420	UniProt ID: Q86SG3	n/a

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Protocols Online

Can sc-100705: DAZ (Z6Q) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp

Thank you for your question. Yes, sc-100705: DAZ (Z6Q) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 5 out of 5 by SCBT QC Lab from Good for Western Blot Antibody detects a band in Daoy whole cell lysate. -SCBT QC

Date published: 2023-09-14

Rated 5 out of 5 by Candela from Published for IHC in human testis This antibody is it has been excellent for IHC for human spermatagonial cells. Try 1:100 dilution for 1h at room temperature, followed by standard protocol of immunohistochemical staining with ABC kit. Definitely purchasing!

Date published: 2021-07-20

Rated 3 out of 5 by JM from Acceptable immunoperoxidase cytoplasmic staining Acceptable immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human testis tissue. -SCBT QC

Date published: 2015-02-13

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DAZ 抗体 (Z6Q): sc-100705

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References:

Can sc-100705: DAZ (Z6Q) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?