DAPK2 抗体 (X15): sc-100371

DAPK2 Antibody (X15) is an IgG₁ κ mouse monoclonal DAPK2 antibody (also designated DAPK2 antibody) suitable for the detection of the DAPK2 protein of human origin by WB, IP and ELISA. DAPK2 Antibody (X15) is available as the non-conjugated anti-DAPK2 antibody. Death-associated protein kinase 2 (DAPK2), also designated death-associated protein kinase-related protein-1 (DRP-1), is a calcium/calmodulin-regulated serine/threonine kinase that binds to calmodulin, undergoes autophosphorylation in response to an increase in cellular calcium concentration, and phosphorylated myosin light chain (MLC) as an exogenous substrate. DAPK2 is expressed in heart, lung, and skeletal muscle and is localized to the cytoplasm. DAPK2 displays significant homology to DAP-kinase, which mediates interferon (IFN)-g-induced apoptosis in HeLa. Subsequently, DAPK2 is thought to function as a possible tumor suppressor gene.

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References:

1. A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK.  |  Jin, Y., et al. 2002. J Biol Chem. 277: 46980-6. PMID: 12351649
2. DAPK catalytic activity in the hippocampus increases during the recovery phase in an animal model of brain hypoxic-ischemic injury.  |  Schumacher, AM., et al. 2002. Biochim Biophys Acta. 1600: 128-37. PMID: 12445468
3. Frequent promoter methylation of CDH1, DAPK, RARB, and HIC1 genes in carcinoma of cervix uteri: its relationship to clinical outcome.  |  Narayan, G., et al. 2003. Mol Cancer. 2: 24. PMID: 12773202
4. Expression of a novel type I keratin, DAPK-1 in the dorsal aorta and pronephric duct of the zebrafish embryos.  |  Jang, WS., et al. 2003. Gene. 312: 145-50. PMID: 12909350
5. Promoter methylation and down-regulation of DAPK is associated with gastric atrophy.  |  Kim, WS., et al. 2003. Int J Mol Med. 12: 827-30. PMID: 14612952
6. Bidirectional signals transduced by DAPK-ERK interaction promote the apoptotic effect of DAPK.  |  Chen, CH., et al. 2005. EMBO J. 24: 294-304. PMID: 15616583
7. Assignment of DAP1 and DAPK--genes that positively mediate programmed cell death triggered by IFN-gamma--to chromosome regions 5p12.2 and 9q34.1, respectively.  |  Feinstein, E., et al. 1995. Genomics. 29: 305-7. PMID: 8530096
8. DAP kinase links the control of apoptosis to metastasis.  |  Inbal, B., et al. 1997. Nature. 390: 180-4. PMID: 9367156
9. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis.  |  Kawai, T., et al. 1998. Mol Cell Biol. 18: 1642-51. PMID: 9488481
10. Molecular cloning and developmental expression of a rat homologue of death-associated protein kinase in the nervous system.  |  Sakagami, H. and Kondo, H. 1997. Brain Res Mol Brain Res. 52: 249-56. PMID: 9495546