CYP8B1抗体 (M15-P3B7) 推荐用于 mouse, rat 和 human 来源的CYP8B1 WB, IP 和 ELISA检测

CYP8B1 抗体 (M15-P3B7): sc-101387

CYP8B1抗体 (M15-P3B7) is rated 3.8 out of 5 by 4.
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Datasheets

    • 抗-CYP8B1 抗体 M15-P3B7 是小鼠单克隆 IgG2b κ,CYP8B1抗体, 在5篇文献中引用,规格为200 µg/ml
    • 免疫合成肽,对应于human来源CYP8B1 486-494氨基酸
    • 抗-CYP8B1 抗体 (M15-P3B7) 推荐用于 WB, IP 和 ELISA,检测mouse, rat 和human 来源的 CYP8B1
    • 抗-CYP8B1 抗体 (M15-P3B7) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取CYP8B1 (M15-P3B7): sc-101387免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Hi, I am a researcher at City of Hope Medical Center. These day we start to have a research at cyp8b1 knockout mice and need a antibody of cyp8b1, and before that can we receive a FREE 10 µg sample of CYP8B1 (M15-P3B7): sc-101387? Appreciate a lot.

Asked by: Blake
Thank you for the question and your interest in our products. We will contact you by email regarding your request.
Answered by: Technical Support Europe
Date published: 2018-02-02

Are there any issues with using sc-101387: CYP8B1 (M15-P3B7) mouse monoclonal primary antibody on mouse samples?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-101387 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 1 out of 5 by from Unspecific bands We got CYP8b1 and FGFR4 antibody samples and they do not work for WB application. For both these antibodies tried on murine liver samples (on different blots) we got unspecific bands at exact same MW which we could not get rid off even after harsh stripping. It took us lot of time and energy for nothing in the end.
Date published: 2018-05-11
Rated 5 out of 5 by from Cyp8b1 antibody gives good bands on Liver Western I received Cyp8b1 sample antibody with Cyp27a1 sample antibody and both gave good bands on mouse liver westerns. Used 1:1000 dilutions for primary and secondary antibodies, and "femto" enhancer instead of regular "pico" enhancer. Got really good bands, see picture
Date published: 2017-09-21
Rated 4 out of 5 by from WB Results I applied for a sample two week ago.My sample is the mouse liver. Incubated the antibody for overnight at 4℃, 1:1000 dilution, and  m-IgGκ BP-HRP  incubated for 1 hour at RT , 1:1000 dilution.The results are shown in the figure.
Date published: 2017-09-16
Rated 5 out of 5 by from WB positive in human liver microsomes WB positive in human liver microsomes. -SCBT QC
Date published: 2013-07-08
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