CYP4V2抗体 (M29-P3B10) 推荐用于 human 来源的CYP4V2 WB, IP 和 ELISA检测

CYP4V2 抗体 (M29-P3B10): sc-101386

CYP4V2抗体 (M29-P3B10) is rated 4.8 out of 5 by 5.
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Datasheets

    • 抗-CYP4V2 抗体 M29-P3B10 是小鼠单克隆 IgG1 (kappa轻链),CYP4V2抗体,规格为200 µg/ml
    • 免疫合成肽,对应于human来源CYP4V2 495-504氨基酸
    • CYP4V2 抗体 (M29-P3B10) 推荐用于WB, IP 和 ELISA,检测human 来源的 CYP4V2
    • 抗-CYP4V2 抗体 (M29-P3B10) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取CYP4V2 (M29-P3B10): sc-101386免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 CYP4V2 Antibody (M29-P3B10) for WB 的首选应用二抗。这些试剂现在以套装形式提供CYP4V2 Antibody (M29-P3B10)。 (见下方订购信息).
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请教个问题:CYP4V2 抗体 (M29-P3B10): sc-101386,该抗体可以检测小鼠的同源蛋白CYP4V3吗?

Asked by: 那年天空
感谢您的提问。CYP4V2 抗体 (M29-P3B10) 推荐用于WB, IP 和 ELISA,检测human 来源的 CYP4V2。对于小鼠CYP4V3蛋白,该抗体没有检测过,故不推荐。 Thank you for your question. CYP4V2 antibody (M29-P3B10) is recommended for detection of CYP4V2 of human origin by WB, IP and ELISA. We didn't test this antibody on mouse CYP4V3 protein, so it is not recommended for detection of mouse CYP4V3 protein.
Answered by: Technical Service SH
Date published: 2022-02-28

What is the recommended starting dilution for Western blot for sc-101386: CYP4V2 (M29-P3B10) monoclonal antibody?

Asked by: Trav11
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Worked very well Worked very well at suggest dilution 1:1000. 1-2 hour incubated primary antibody,blocking use 5% BSA, no background.
Date published: 2017-09-02
Rated 5 out of 5 by from Recommended use. Used this antibody for WB detection and it worked well,recommended use.
Date published: 2017-04-28
Rated 5 out of 5 by from the result is expected. Band in the work found in a large number of cell lines, the result is expected.
Date published: 2017-02-11
Rated 5 out of 5 by from Powerful band Powerful band detected in a large number of cell lines, the result satisfied.
Date published: 2016-12-10
Rated 4 out of 5 by from Good detection of CYP4V2 with human liver Good detection of CYP4V2 with human liver microsomes in WB -SCBT QC
Date published: 2014-12-21
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