CYP1A1 抗体 (1A3-03): sc-101828

4.3(3)

写一个评价提问

Datasheets

- CYP1A1抗体(1A3-03)是小鼠单克隆IgG₁ κ, 在4篇文献中引用，规格为200 µg/ml
- raised against CYP1A1 isolated from 3-methylcholanthrene treated microsomes of rat liver origin
- 抗-CYP1A1 抗体 (1A3-03) 推荐用于 WB, IP, IF, FCM 和 ELISA，检测mouse, rat, human 和porcine 来源的 CYP1A1; 不会与 CYP1A2产生交联反应
- 抗-CYP1A1 抗体 (1A3-03) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
- 还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
- 还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
- 关于如何获取CYP1A1 (1A3-03): sc-101828的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP and m-IgG₁ BP-HRP 是 CYP1A1 Antibody (1A3-03) for WB首选应用二抗。这些试剂现在以套装形式提供CYP1A1 Antibody (1A3-03) (见下方订购信息).

SEE ALSO...

此外 CYP 抗体包括 CYP11B, CYP17A1, CYP19, CYP1A1, CYP1A2, CYP1B1, CYP24, CYP26A1, CYP27A1和 CYP27B1
ChemCruz™ Chemical CYP Inhibitors or CYP Substrates可用于CYP细胞反应的功能性分析

CYP1A1 Antibody (1A3-03) - Western Blotting - Image 21846

CYP1A1 Antibody (1A3-03) - Western Blotting - Image 59237

点击图片或放大按钮来放大

CYP1A1 Antibody (1A3-03) is an IgG1 κ mouse monoclonal CYP1A1 antibody that detects CYP1A1 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA. CYP1A1 Antibody (1A3-03) is available as the non-conjugated anti-CYP1A1 antibody form, as well as multiple conjugated forms of anti-CYP1A1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. NADPH cytochrome P450 reductase is a microsomal enzyme responsible for the transfer of electrons from NADPH to cytochrome P450 enzymes during the P450 catalytic cycle. NADPH cytochrome P450 reductase is localized to the endoplasmic reticulum where it is also able to transfer electrons to heme oxygenase and cytochrome b5. NADPH cytochrome P450 reductase is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase (FNR) and flavodoxin. Electron transfer of NADPH cytochrome P450 reductase requires the binding of two flavin cofactors, FAD and FMN, to the FNR and flavodoxin domains, respectively.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	CYP1A1	Entrez Gene ID: 1543	15q24.1	XM_017021953, NM_000499, NM_001319216, NM_001319217	UniProt ID: P04798	108330
Mouse	Cyp1a1	Entrez Gene ID: 13076	9 B	XM_006510810, NM_001136059, NM_009992	UniProt ID: P00184	N/A

Download Datasheet

Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗CYP1A1抗体(1A3-03)在各种科学出版物中有4篇引文。

针对哺乳动物靶标蛋白的一抗，如抗CYP1A1 抗体 (1A3-03)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

CYP1A1 抗体 (1A3-03) Specifications

替代产品名称

CYP1A1

产品克隆名称

1A3-03

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

抗原表位靶标

CYP1A1

抗原表位种属

rat

产品反应性/检测

CYP1A1

种属反应性/检测

mouse, rat, human, porcine

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, FCM, ELISA

瓶的信息

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

4篇各种科学出版物的引文

可选的偶联物/形式

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase PE: Phycoerythrin

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗CYP1A1抗体(1A3-03)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), FCM (Flow Cytometry), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

優選的 CYP1A1 抗體是 CYP1A1 抗體 (B-4): sc-25304.

Can CYP1A1 (1A3-03): sc-101828 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CYP1A1 (1A3-03): sc-101828 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by MuGu from Works great! Worked great for human and mouse tissue lysates when used at 1:200.

Date published: 2018-03-23

Rated 5 out of 5 by Randy McDonald from positive western blot We used this antibody for a western blot experiment and got positive bands at the correct molecular weight

Date published: 2017-01-18

Rated 3 out of 5 by ET from Worked with human transfected lysate by WB Worked with human transfected lysate by WB -SCBT QC

Date published: 2014-11-24

选择店铺

选择语言

选择币种

CYP1A1 抗体 (1A3-03): sc-101828

QUICK LINKS

SEE ALSO...

CYP1A1 抗体 (1A3-03) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

優選的 CYP1A1 抗體是 CYP1A1 抗體 (B-4): sc-25304.

Can CYP1A1 (1A3-03): sc-101828 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?