CRY1抗体 (W-L5) 推荐用于 mouse, rat 和 human 来源的CRY1 WB, IP, IF, IHC(P) 和 ELISA检测

CRY1 抗体 (W-L5): sc-101006

CRY1抗体 (W-L5) is rated 3.9 out of 5 by 8.
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Datasheets

    • CRY1抗体(W-L5)是小鼠单克隆IgG1 (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组CRY1
    • 推荐用于 mouse, rat 和 human 来源的CRY1 WB, IP, IF, IHC(P) 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取CRY1 (W-L5): sc-101006免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Can sc-101006: CRY1 (W-L5) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, sc-101006: CRY1 (W-L5) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24

I have been having problems producing clean blots with specific bands with this antibody. Is anyone here willing to share their blotting procedure? Thanks in advance!

Asked by: Tyrsett
Thank you for your question. We recommend blocking with Blotto (5% nonfat milk in TBST) for one hour at room temperature, but you can try overnight at 4C if you are experiencing high background. We then recommend diluting the primary antibody in fresh Blotto at a dilution of 1:200 and incubating for one hour at room temperature. We load 50 µg sample protein. And to help reduce non-specific banding, we recommend using our specific mouse IgG kappa binding protein (sc-516102) in place of a conventional anti-mouse IgG secondary antibody. A link to our complete recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting If you continue to have trouble, please contact our Technical Service Department and we can assist you with troubleshooting the antibody.
Answered by: Technical Support
Date published: 2017-03-13
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Rated 1 out of 5 by from barely detectable by WB I have tested this antibody WB of mouse hippocampus and could only observe an extremely faint band of the expected size, after long exposure and high antibody concentration
Date published: 2018-10-24
Rated 5 out of 5 by from perfect result Cry1 is a circadian clock gene,we use this antibody got the Conclusion :Cry1 is increased in poorly differentiated adenocarcinoma,It's very statisfied result use 1:800.
Date published: 2017-07-15
Rated 5 out of 5 by from Good product The CRY1 Antibody (W-L5): sc-101006 works well for IHC applications with formalin fixed, paraffin-embedded tissue sections showing staining pattern localized to the nucleus and cytoplasm.
Date published: 2017-02-03
Rated 4 out of 5 by from Solid WB signal with a minimum of non Solid WB signal with a minimum of non-specific bands!
Date published: 2015-11-16
Rated 4 out of 5 by from Band detected at expected mw in HeLa nuclear Band detected at expected mw in HeLa nuclear extract. -SCBT QC
Date published: 2015-05-04
Rated 4 out of 5 by from Produced great immunoperoxidase nuclear and Produced great immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue. -SCBT QC
Date published: 2015-04-29
Rated 4 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using human CRY1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-22
Rated 4 out of 5 by from Clear Clear, specific band observed in WB in mouse CRY1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-01-25
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