CRY1 抗体 (W-L5): sc-101006

3.9(8)

- CRY1抗体(W-L5)是小鼠单克隆IgG₁ κ, 在5篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组CRY1
- 推荐用于 mouse, rat 和 human 来源的CRY1 WB, IP, IF, IHC(P) 和 ELISA检测
- 关于如何获取CRY1 (W-L5): sc-101006的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP and m-IgG₁ BP-HRP 是 CRY1 Antibody (W-L5) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供CRY1 Antibody (W-L5) (见下方订购信息).

References:

Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2. | Vitaterna, MH., et al. 1999. Proc Natl Acad Sci U S A. 96: 12114-9. PMID: 10518585
Light-independent role of CRY1 and CRY2 in the mammalian circadian clock. | Griffin, EA., et al. 1999. Science. 286: 768-71. PMID: 10531061
A 24-hour circadian clock is found in the mammalian retina. | Morell, V. 1996. Science. 272: 349. PMID: 8602520
A differential response of two putative mammalian circadian regulators, mper1 and mper2, to light. | Albrecht, U., et al. 1997. Cell. 91: 1055-64. PMID: 9428527
Mammalian circadian autoregulatory loop: a timeless ortholog and mPer1 interact and negatively regulate CLOCK-BMAL1-induced transcription. | Sangoram, AM., et al. 1998. Neuron. 21: 1101-13. PMID: 9856465
Molecular analysis of mammalian timeless. | Zylka, MJ., et al. 1998. Neuron. 21: 1115-22. PMID: 9856466
Molecular bases for circadian clocks. | Dunlap, JC. 1999. Cell. 96: 271-90. PMID: 9988221
A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clock. | Jin, X., et al. 1999. Cell. 96: 57-68. PMID: 9989497

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	CRY1	Entrez Gene ID: 1407	12q23.3	XM_017018832, NM_004075	UniProt ID: Q16526	601933
Mouse	Cry1	Entrez Gene ID: 12952	10 C1	XM_011243348, XM_011243349, NM_007771	UniProt ID: P97784	N/A

Download Datasheet

Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗CRY1抗体(W-L5)在各种科学出版物中有5篇引文。

针对哺乳动物靶标蛋白的一抗，如抗CRY1 抗体 (W-L5)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

CRY1 抗体 (W-L5) Specifications

替代产品名称

Cryptochrome-1

产品克隆名称

W-L5

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

1-587

抗原表位靶标

CRY1

抗原表位种属

human

产品反应性/检测

CRY1

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

5篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗CRY1抗体(W-L5)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

優選的 CRY1 抗體是 CRY1 抗體 (H-12): sc-393466.

Can sc-101006: CRY1 (W-L5) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine

Thank you for your question. Yes, sc-101006: CRY1 (W-L5) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-03-24

I have been having problems producing clean blots with specific bands with this antibody. Is anyone here willing to share their blotting procedure? Thanks in advance!

Asked by: Tyrsett

Thank you for your question. We recommend blocking with Blotto (5% nonfat milk in TBST) for one hour at room temperature, but you can try overnight at 4C if you are experiencing high background. We then recommend diluting the primary antibody in fresh Blotto at a dilution of 1:200 and incubating for one hour at room temperature. We load 50 µg sample protein. And to help reduce non-specific banding, we recommend using our specific mouse IgG kappa binding protein (sc-516102) in place of a conventional anti-mouse IgG secondary antibody. A link to our complete recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting If you continue to have trouble, please contact our Technical Service Department and we can assist you with troubleshooting the antibody.

Answered by: Technical Support

Date published: 2021-01-05

Rated 1 out of 5 by JoanaFig from barely detectable by WB I have tested this antibody WB of mouse hippocampus and could only observe an extremely faint band of the expected size, after long exposure and high antibody concentration

Date published: 2018-10-24

Rated 5 out of 5 by suyan from perfect result Cry1 is a circadian clock gene,we use this antibody got the Conclusion :Cry1 is increased in poorly differentiated adenocarcinoma,It's very statisfied result use 1:800.

Date published: 2017-07-15

Rated 5 out of 5 by Trav11 from Good product The CRY1 Antibody (W-L5): sc-101006 works well for IHC applications with formalin fixed, paraffin-embedded tissue sections showing staining pattern localized to the nucleus and cytoplasm.

Date published: 2017-02-03

Rated 4 out of 5 by Xin_Tong from Solid WB signal with a minimum of non Solid WB signal with a minimum of non-specific bands!

Date published: 2015-11-16

Rated 4 out of 5 by KG from Band detected at expected mw in HeLa nuclear Band detected at expected mw in HeLa nuclear extract. -SCBT QC

Date published: 2015-05-04

Rated 4 out of 5 by FL from Produced great immunoperoxidase nuclear and Produced great immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue. -SCBT QC

Date published: 2015-04-29

Rated 4 out of 5 by MS from Clean blot with single strong band at expected Clean blot with single strong band at expected size using human CRY1 transfected 293T whole cell lysate. -SCBT QC

Date published: 2015-03-22

Rated 4 out of 5 by JL from Clear Clear, specific band observed in WB in mouse CRY1 transfected 293T whole cell lysate. -SCBT QC

Date published: 2015-01-25

选择店铺

选择语言

选择币种