CREM抗体 (22) 推荐用于 human 来源的CREM WB, IF, IHC(P) 和 ELISA检测

CREM 抗体 (22): sc-101530

CREM抗体 (22) is rated 3.5 out of 5 by 2.
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Datasheets

    • 抗-CREM 抗体 22 是小鼠单克隆 IgG1 (kappa轻链),CREM抗体,规格为200 µg/ml
    • 免疫human物种的重组CREM
    • CREM 抗体 (22) 推荐用于WB, IF, IHC(P) 和 ELISA,检测human 来源的 CREM
    • 抗-CREM 抗体 (22) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取CREM (22): sc-101530免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

How can CREM (22): sc-101530 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-101530 HRP), phycoerythrin (sc-101530 PE), fluoroscein (sc-101530 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Specific for CREM in WB I overexpressed GFP-tagged ATF1, CREB and CREM in HEK293 cells. CREM 22 antibody was very specific to CREM in WB (dilution 1:200), as it recognised only GFP-CREM protein, but not GFP-ATF1 or GFP-CREB. On the other hand, I could not detect endogenous levels of CREM in rat primary cortical neurons with this antibody.
Date published: 2018-08-23
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